Anti-GABA A Receptor beta 3/GABRB3 抗体 (ab104659)
Key features and details
- Rabbit polyclonal to GABA A Receptor beta 3/GABRB3
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-GABA A Receptor beta 3/GABRB3 antibody
GABA A Receptor beta 3/GABRB3 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to GABA A Receptor beta 3/GABRB3 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: IHC-P -
種交差性
交差種: Mouse, Rat, Human
交差が予測される動物種: Rabbit -
免疫原
Synthetic peptide corresponding to Rat GABA A Receptor beta 3/GABRB3 aa 400 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available asab125892) -
ポジティブ・コントロール
- WB: Rat and mouse brain and hippocampus tissues.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab104659の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa).
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特記事項 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 54 kDa (predicted molecular weight: 54 kDa). |
ターゲット情報
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機能
Component of the heteropentameric receptor for GABA, the major inhibitory neurotransmitter in the vertebrate brain. Functions also as histamine receptor and mediates cellular responses to histamine. Functions as receptor for diazepines and various anesthetics, such as pentobarbital; these are bound at a separate allosteric effector binding site. Functions as ligand-gated chloride channel. -
関連疾患
Epilepsy, childhood absence 5 -
配列類似性
Belongs to the ligand-gated ion channel (TC 1.A.9) family. Gamma-aminobutyric acid receptor (TC 1.A.9.5) subfamily. GABRB3 sub-subfamily. -
細胞内局在
Cell junction, synapse, postsynaptic cell membrane. Cell membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 2562 Human
- Entrez Gene: 14402 Mouse
- Entrez Gene: 24922 Rat
- Omim: 137192 Human
- SwissProt: P28472 Human
- SwissProt: P63080 Mouse
- SwissProt: P63079 Rat
- Unigene: 302352 Human
see all -
別名
- ECA5 antibody
- GABA alpha receptor beta-2 subunit antibody
- GABA(A) receptor subunit beta-3 antibody
see all
画像
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All lanes : Anti-GABA A Receptor beta 3/GABRB3 antibody (ab104659) at 1 µg/ml
Lane 1 : Brain (Rat) Tissue Lysate
Lane 2 : Brain (Mouse) Tissue Lysate
Lane 3 : Rat Hippocampus Tissue Lysate
Lane 4 : Mouse Hippocampus Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 54 kDa
Observed band size: 58 kDa why is the actual band size different from the predicted?
Additional bands at: 40 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 secondsGABA A Receptor beta 3/GABRB3 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
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All lanes : Anti-GABA A Receptor beta 3/GABRB3 antibody (ab104659) at 1 µg/ml
Lane 1 : Rat Brain Tissue Lysate
Lane 2 : Mouse Brain Tissue Lysate
Lane 3 : Human brain tissue lysate
Lane 4 : Rat Hippocampus Tissue Lysate
Lane 5 : Mouse Hippocampus Tissue Lysate
Lane 6 : Human hippocampus Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 54 kDa
Exposure time: 4 minutes
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab104659 は 1 報の論文で使用されています。
- Oda R et al. Direct visualization of general anesthetic propofol on neurons by stimulated Raman scattering microscopy. iScience 25:103936 (2022). PubMed: 35252821