Recombinant human Casein Kinase 1 delta/CSNK1D protein (ab103955)
Key features and details
- Expression system: Baculovirus infected Sf9 cells
- Purity: > 80% Densitometry
- Active: Yes
- Suitable for: Functional Studies, SDS-PAGE, WB
製品の詳細
-
製品名
Recombinant human Casein Kinase 1 delta/CSNK1D protein
Casein Kinase 1 delta/CSNK1D タンパク質・ペプチド 製品一覧 -
生理活性
The Specific activity of ab103955 was determined to be 82 nmol/min/mg.
-
精製度
> 80 % Densitometry.
Purity was determined to be >80% by densitometry. Affinity purified. -
発現系
Baculovirus infected Sf9 cells -
アクセッション番号
-
タンパク質長
Full length protein -
Animal free
No -
由来
Recombinant -
-
生物種
Human -
予測される分子量
72 kDa including tags -
領域
1 to 415
-
関連製品
-
Related Products
特性
Our Abpromise guarantee covers the use of ab103955 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
アプリケーション
Functional Studies
SDS-PAGE
Western blot
-
製品の状態
Liquid -
備考
ab91090 (Cow Casein full length protein) can be utilized as a substrate for assessing Kinase activity
Previously labelled as Casein Kinase 1 delta.
-
Concentration information loading...
前処理および保存
-
保存方法および安定性
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
pH: 7.50
Constituents: 0.307% Glutathione, 0.00174% PMSF, 0.00385% DTT, 0.79% Tris HCl, 0.00292% EDTA, 25% Glycerol (glycerin, glycerine), 0.87% Sodium chlorideThis product is an active protein and may elicit a biological response in vivo, handle with caution.
関連情報
-
別名
- Casein Kinase 1 delta
- Casein kinase I delta isoform
- Casein kinase I isoform delta
see all -
機能
Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Central component of the circadian clock. May act as a negative regulator of circadian rhythmicity by phosphorylating PER1 and PER2. Retains PER1 in the cytoplasm. -
組織特異性
Expressed in all tissues examined, including brain, heart, lung, liver, pancreas, kidney, placenta and skeletal muscle. In blood, highly expressed in hemopoietic cells and mature granulocytes. Also found in monocytes and lymphocytes. -
関連疾患
Defects in CSNK1D are a cause of familial advanced sleep-phase syndrome (FASPS) [MIM:604348]. FASPS is characterized by very early sleep onset and offset. Individuals are 'morning larks' with a 4 hours advance of the sleep, temperature and melatonin rhythms. -
配列類似性
Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. Casein kinase I subfamily.
Contains 1 protein kinase domain. -
翻訳後修飾
Autophosphorylated on serine and threonine residues. -
細胞内局在
Cytoplasm. Nucleus. - Information by UniProt
画像
-
The specific activity of Casein Kinase 1 delta/CSNK1D (ab103955) was determined to be 77 nmol/min/mg as per activity assay protocol
-
SDS PAGE analysis of ab103955
-
SDS PAGE analysis of ab103955
-
The Specific activity of ab103955 was determined to be 82 nmol/min/mg.
-
SDS-PAGE showing ab103955 at approximately 72kDa.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (2)
ab103955 は 2 報の論文で使用されています。
- Ibrahim H et al. Phosphorylation of GAPVD1 Is Regulated by the PER Complex and Linked to GAPVD1 Degradation. Int J Mol Sci 22:N/A (2021). PubMed: 33917494
- Hanna-Addams S et al. CK1a, CK1d, and CK1e are necrosome components which phosphorylate serine 227 of human RIPK3 to activate necroptosis. Proc Natl Acad Sci U S A 117:1962-1970 (2020). PubMed: 31932442