製品の概要

  • 製品名Anti-AATF antibody
    AATF 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to AATF
  • アプリケーション適用あり: WB, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chimpanzee, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region between residues 125 and 175 of human AATF (NP_036270.1).

  • ポジティブ・コントロール
    • HeLa whole cell lysate.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab72148 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Detects a band of approximately 80 kDa (predicted molecular weight: 62 kDa).
IP Use at 2-5 µg/mg of lysate.

ターゲット情報

  • 機能May function as a general inhibitor of the histone deacetylase HDAC1. Binding to the pocket region of RB1 may displace HDAC1 from RB1/E2F complexes, leading to activation of E2F target genes and cell cycle progression. Conversely, displacement of HDAC1 from SP1 bound to the CDKN1A promoter leads to increased expression of this CDK inhibitor and blocks cell cycle progression. Also antagonizes PAWR mediated induction of aberrant amyloid peptide production in Alzheimer disease (presenile and senile dementia), although the molecular basis for this phenomenon has not been described to date.
  • 組織特異性Ubiquitously expressed. Expressed at high levels in brain, heart, kidney, placenta and thymus.
  • 配列類似性Belongs to the AATF family.
  • 翻訳後修飾Hyperphosphorylated during the G1/S phase transition.
  • 細胞内局在Nucleus > nucleolus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AATF antibody
    • AATF_HUMAN antibody
    • Apoptosis antagonizing transcription factor antibody
    • Apoptosis-antagonizing transcription factor antibody
    • CHE 1 antibody
    • CHE1 antibody
    • DED antibody
    • Protein AATF antibody
    • Rb binding protein Che 1 antibody
    • Rb-binding protein Che-1 antibody
    see all

Anti-AATF antibody 画像

  • All lanes : Anti-AATF antibody (ab72148) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg


    Predicted band size : 62 kDa
    Observed band size : 80 kDa (why is the actual band size different from the predicted?)
  • Detection of Human AATF by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded), using ab72148 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection.

Anti-AATF antibody (ab72148) 使用論文

ab72148 has not yet been referenced specifically in any publications.

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