Anti-AATF/DED 抗体 [3C7] (ab219073)
Key features and details
- Mouse monoclonal [3C7] to AATF/DED
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-AATF/DED antibody [3C7]
AATF/DED 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [3C7] to AATF/DED -
由来種
Mouse -
アプリケーション
適用あり: ICC/IF, WB, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment (GST-tag) corresponding to Human AATF/DED aa 150-250. NCBI Accession No. AAH00591.
Database link: Q9NY61 -
ポジティブ・コントロール
- Human kidney tissue; HeLa cells; HeLa cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
3C7 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab219073の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
Use a concentration of 10 µg/ml.
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 63 kDa.
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IHC-P |
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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特記事項 |
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ICC/IF
Use a concentration of 10 µg/ml. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 63 kDa. |
IHC-P
Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ターゲット情報
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機能
May function as a general inhibitor of the histone deacetylase HDAC1. Binding to the pocket region of RB1 may displace HDAC1 from RB1/E2F complexes, leading to activation of E2F target genes and cell cycle progression. Conversely, displacement of HDAC1 from SP1 bound to the CDKN1A promoter leads to increased expression of this CDK inhibitor and blocks cell cycle progression. Also antagonizes PAWR mediated induction of aberrant amyloid peptide production in Alzheimer disease (presenile and senile dementia), although the molecular basis for this phenomenon has not been described to date. -
組織特異性
Ubiquitously expressed. Expressed at high levels in brain, heart, kidney, placenta and thymus. -
配列類似性
Belongs to the AATF family. -
翻訳後修飾
Hyperphosphorylated during the G1/S phase transition. -
細胞内局在
Nucleus > nucleolus. - Information by UniProt
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参照データベース
- Entrez Gene: 26574 Human
- Omim: 608463 Human
- SwissProt: Q9NY61 Human
- Unigene: 195740 Human
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別名
- AATF antibody
- AATF_HUMAN antibody
- Apoptosis antagonizing transcription factor antibody
see all
画像
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Immunofluorescent analysis of HeLa cells labeling AATF/DED using ab219073 at 10 µg/mL.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-AATF/DED antibody [3C7] (ab219073)
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue labeling AATF/DED using ab219073 at 10 µg/ml.
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Anti-AATF/DED antibody [3C7] (ab219073) + HeLa cell lysate
Predicted band size: 63 kDa
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (0)
ab219073 は論文での使用が確認できていません。