Anti-AADACL1 抗体 (ab111544)

製品の概要

  • 製品名Anti-AADACL1 antibody
  • 製品の詳細
    Rabbit polyclonal to AADACL1
  • アプリケーション適用あり: ICC/IF, WB, IHC-Fr, ICCmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within internal amino acids 224-273 (LQALDFNTPS YQQSMNTPIL PRHVMVRYWL DYFKGNYDFV EAMIVNNHTS) of Mouse AADACL1 (NP_848887).

  • ポジティブ・コントロール
    • Mouse kidney lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab111544 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 0.2 - 1 µg/ml. Predicted molecular weight: 46 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-Fr Use at an assay dependent concentration.
ICC 1/200.

ターゲット情報

  • 機能Hydrolyzes 2-acetyl monoalkylglycerol ether, the penultimate precursor of the pathway for de novo synthesis of platelet-activating factor. May be responsible for cholesterol ester hydrolysis in macrophages, thereby contributing to the development of atherosclerosis. Also involved in organ detoxification by hydrolyzing exogenous organophosphorus compounds. May contribute to cancer pathogenesis by promoting tumor cell migration.
  • 組織特異性Expressed in monocyte-derived macrophages. Up-regulated in invasive melanoma and breast carcinoma cell lines.
  • 配列類似性Belongs to the 'GDXG' lipolytic enzyme family.
  • 翻訳後修飾N-glycosylated.
  • 細胞内局在Membrane. Microsome.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Arylacetamide deacetylase like 1 antibody
    • Arylacetamide deacetylase-like 1 antibody
    • NCEH antibody
    • Nceh1 antibody
    • NCEH1_HUMAN antibody
    • Neutral cholesterol ester hydrolase 1 antibody
    • Neutral cholesterol ester hydrolase antibody
    see all

Anti-AADACL1 antibody 画像

  • Anti-AADACL1 antibody (ab111544) at 1 µg/ml + Mouse kidney lysate at 10 µg

    Predicted band size : 46 kDa
    Gel concentration: 12%
  • ab114544 staining AADACL1 in cultured human prostate cells by ICC (Immunocytochemistry). Cells were fixed with methanol and blocked with 1% BSA for 24 hours at 4°C. Samples were incubated with primary antibody (1/200 in blocking buffer) for 2 hours at 37°C. A Biotin-conjugated Goat anti-rabbit polyclonal (1/200) was used as the secondary antibody.

    See Abreview

  • ab111544 staining AADACL1 in Human Prostate cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 1% BSA + 2% serum for 24 hours at 4°C. Samples were incubated with primary antibody (1/200 in blocking agent) for 2 hours at 37°C. An undiluted FITC-conjugated Goat anti-rabbit IgG polyclonal was used as the secondary antibody.

    See Abreview

Anti-AADACL1 antibody (ab111544) 使用論文

ab111544 has not yet been referenced specifically in any publications.

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (Prostate tumour)
Specification Prostate tumour
Fixative Methanol
Permeabilization No
Blocking step BSA as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Username

Abcam user community

Verified customer

投稿 Aug 10 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Prostate)
Specification Prostate
Fixative Paraformaldehyde
Permeabilization No
Blocking step BSA as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Username

Abcam user community

Verified customer

投稿 Jun 08 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry
Sample Human Cultured Cells (Prostate)
Specification Prostate
Fixative Methanol
Permeabilization No
Blocking step BSA as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Username

Dr. Olayinka Oloko

Verified customer

投稿 Apr 09 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (prostate)
Loading amount 25 µg
Specification prostate
Gel Running Conditions Reduced Denaturing (10% gel)
Blocking step Milk as blocking agent for 24 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Dr. Olayinka Oloko

Verified customer

投稿 Dec 07 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"