A431 nuclear extract lysate (EGF treated) (ab14635)

製品の概要

  • 製品名
    A431 nuclear extract lysate (EGF treated)
    A431 ライゼート 製品一覧
  • 製品の詳細
    A431 nuclear extract lysate
  • 特記事項
    Cell line: A431 (Human epithelial carcinoma).
    Cells were cultured in medium supplemented with 10 ng/mL EGF (epidermal growth factor) for 10 minutes at 37°C immediately prior to harvesting.
    We recommend aliquoting the extracts into single use fractions and then storing.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 20% Glycerol, 20mM HEPES, 100mM Potassium Chloride, 1mM Magnesium Chloride, 0.5mM PMSF, 0.5mM DTT, 10 ng/mL EGF (epidermal growth factor). pH 7.9
  • Concentration information loading...
  • 研究分野
  • 背景
    A431 cells are a positive control for EGFR as they express high levels of EGFR. They also used for cell cycle studies and can be stimulated with EGF to stimulate EGFR and are therefore a very useful model to study EGFR downstream signalling cascades eg shc, src, MAPK.

参考文献

ab14635 has not yet been referenced specifically in any publications.

レビューと Q&A

J'ai mis en place l'envoi d'une unité gratuite de lysat de A431 (Human epithelial carcinoma cell line) Nuclear Lysate - EGF treated, référence ab14635. Le numéro de commande est : *****.

Ce lysat est recommand&eacu...

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Thank you for your enquiry. ab14635 (A431 (Human epithelial carcinoma cell line) Nuclear Lysate - EGF treated) has been quality control tested by Western blot and the Electrophoretic Mobility Shift Assay (EMSA). Positive Transcription Factors in Wes...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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