A431 membrane extract lysate (ab30134)

製品の概要

  • 製品名A431 membrane extract lysate
    A431 ライゼート 製品一覧
  • 特記事項Cell line: A431 (Human epithelial carcinoma).


    Protein is isolated from whole tissue homogenates using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70ºC. Quality control: 1. The isolated protein pattern on SDS-PAGE gel is visualized by coomassie blue staining. The pattern is consistent with each lot. 2. The isolated protein is Western Blot analyzed by either a GAPDH or beta-actin antibody. The expression level is consistent with each lot.

  • アプリケーション適用あり: WBmore details

法規制情報

製品の特性

  • 製品の状態Liquid
  • 保存方法Store at -80°C. Allow to warm to room temp and agitate gently before aliquotting.
  • バッファーPreservative: None
    Constituents: 2% SDS, 10% Glycerol, 0.1% Bromophenol blue, Protease inhibitor, Sodium chloride, EDTA, Magnesium chloride, HEPES, 0.15M Beta mercaptoethanol
  • Concentration information loading...
  • ライゼート 備考Protein is isolated from whole tissue homogenates using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70ºC. Quality control: 1. The isolated protein pattern on SDS-PAGE gel is visualized by coomassie blue staining. The pattern is consistent with each lot. 2. The isolated protein is Western Blot analyzed by either a GAPDH or beta-actin antibody. The expression level is consistent with each lot.
  • 研究分野
  • 背景A431 cells are a positive control for EGFR as they express high levels of EGFR. They also used for cell cycle studies and can be stimulated with EGF to stimulate EGFR and are therefore a very useful model to study EGFR downstream signalling cascades eg shc, src, MAPK.

アプリケーション

Our Abpromise guarantee covers the use of ab30134 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent dilution. Samples need to boiled for 5 minutes and put on ice before use.

A431 membrane extract lysate (ab30134) 使用論文

ab30134 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"