Anti-A2BP1 / Fox1 抗体 (ab83574)

製品の概要

  • 製品名Anti-A2BP1 / Fox1 antibody
    A2BP1 / Fox1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to A2BP1 / Fox1
  • アプリケーション適用あり: WB, ELISA, IHC-FoFrmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Cow
  • 免疫原

    Synthetic peptide from within the N terminal amino acids 1-50: MNCEREQLRG NQEAAAAPDT MAQPYASAQF APPQNGIPAE YTAPHPHPAP, of Human A2BP1/ Fox1 (NP_001135805)

  • ポジティブ・コントロール
    • MCF7 cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab83574 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 43 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1:62500.

IHC-FoFr Use at an assay dependent concentration.

ターゲット情報

  • 機能RNA-binding protein that regulates alternative splicing events by binding to 5'-UGCAUGU-3' elements. Regulates alternative splicing of tissue-specific exons and of differentially spliced exons during erythropoiesis.
  • 組織特異性Predominantly expressed in muscle and brain.
  • 配列類似性Contains 1 RRM (RNA recognition motif) domain.
  • 細胞内局在Nucleus. Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 2BP1 antibody
    • A2 BP1 antibody
    • A2BP 1 antibody
    • A2BP antibody
    • A2BP1 antibody
    • Ataxin 2 binding protein 1 antibody
    • Ataxin-2-binding protein 1 antibody
    • Ataxin2 binding protein 1 antibody
    • FOX 1 antibody
    • Fox-1 homolog A antibody
    • fox-1-like RNA-binding protein 1 antibody
    • Hexaribonucleotide binding protein 1 antibody
    • Hexaribonucleotide-binding protein 1 antibody
    • HRNBP 1 antibody
    • HRNBP1 antibody
    • RBFOX1 antibody
    • RFOX1_HUMAN antibody
    • RNA binding protein fox-1 homolog 1 antibody
    • RNA binding protein fox-1 homolog 1, C. elegans, homolog of, 1 antibody
    • RNA binding protein, fox-1 homolog (C. elegans) 1 antibody
    see all

Anti-A2BP1 / Fox1 antibody 画像

  • Anti-A2BP1 / Fox1 antibody (ab83574) at 1 µg/ml (in 5% skim milk/ PBS) + MCF7 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 43 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.

Anti-A2BP1 / Fox1 antibody (ab83574) 使用論文

ab83574 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting Abcam.

Under our Abpromise, which is valid for 6 months after delivery, we guarantee ab83574 to work in western blot, ELISA & IHC-FoFr on mouse and human tissue. We have gone through a number of different lots of t...

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Thank you for your enquiry regarding our two antibodies (ab83574 and ab94581). Generally, brain (CNS) cells express Fox1 at high levels particularly in the following region cerebral cortex, hippocampus and lateral ventricle. As the datasheets indic...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (Coronal Brain)
Specification Coronal Brain
Fixative Paraformaldehyde
Permeabilization Yes - PBS + 0.1% Tween20
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
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投稿 Feb 24 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"