製品の概要

  • 製品名Anti-A1CF antibody
    A1CF 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to A1CF
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 2-51 (EAVCLGTCPE PEASMSTAIP GLKKGNNALQ SIILQTLLEK ENGQRKYGGP) of human A1CF (NP_620311).

  • ポジティブ・コントロール
    • HepG2 cell lysate

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab83276 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 65 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay, 1:1562500.

ターゲット情報

  • 機能Essential component of the apolipoprotein B mRNA editing enzyme complex which is responsible for the postranscriptional editing of a CAA codon for Gln to a UAA codon for stop in APOB mRNA. Binds to APOB mRNA and is probably responsible for docking the catalytic subunit, APOBEC1, to the mRNA to allow it to deaminate its target cytosine. The complex also protects the edited APOB mRNA from nonsense-mediated decay.
  • 組織特異性Widely expressed with highest levels in brain, liver, pancreas, colon and spleen.
  • 配列類似性Contains 3 RRM (RNA recognition motif) domains.
  • ドメインThe RRM domains are necessary but not sufficient for binding to APOB mRNA. Additional residues in the pre-RRM and C-terminal regions are required for RNA-binding and for complementing APOBEC1 activity.
  • 細胞内局在Nucleus. Endoplasmic reticulum. Cytoplasm. Predominantly nuclear where it localizes to heterochromatin. Also cytoplasmic where it is found at the outer surface of the endoplasmic reticulum (By similarity). Shuttles between the nucleus and cytoplasm. May be transported into the nucleus by the nuclear import protein TNPO2/TRN2 or by APOBEC1.
  • Information by UniProt
  • 参照データベース
  • 別名
    • A1CF antibody
    • A1CF_HUMAN antibody
    • ACF antibody
    • ACF64 antibody
    • ACF65 antibody
    • Apo B RNA editing protein antibody
    • Apobec 1 complementation factor antibody
    • Apobec 1 complementation factor (ACF) (ASP) antibody
    • APOBEC 1 stimulating protein antibody
    • APOBEC1 complementation factor antibody
    • APOBEC1 stimulating protein antibody
    • APOBEC1-stimulating protein antibody
    • APOBEC1CF antibody
    • ASP antibody
    • MGC163391 antibody
    • RP11-564C4.2 antibody
    see all

Anti-A1CF antibody 画像

  • Anti-A1CF antibody (ab83276) at 1 µg/ml + HepG2 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 65 kDa
    Observed band size : 65 kDa

Anti-A1CF antibody (ab83276) 使用論文

ab83276 has not yet been referenced specifically in any publications.

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