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Recombinant fragment: RQQFYHSVQD LSGGSRQHEA PSNRPLNELL TPQGPSPRTQ HCDPEHFPFP APANAPLQRI RSTSTPNVHM VSTTAPMDSN LIQLTGQSFS TDAAGSRGGS , corresponding to amino acids 151-251 of Human A RAF
Our Abpromise guarantee covers the use of ab54827 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 68 kDa.|
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: ProteinX knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: Raji cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab54827 observed at 68 kDa. Red - loading control, ab181602, observed at 37 kDa.
ab54827 was shown to recognize A RAF when A RAF knockout samples were used, along with additional cross-reactive bands. Wild-type and A RAF knockout samples were subjected to SDS-PAGE. ab54827 and ab181602 (loading control to GAPDH) were diluted 1/2000 and 1/10 000 and incubated overnight at 4°C. Blots were developed with Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772 and Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.
ab54827 has not yet been referenced specifically in any publications.