Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Small intestine)
Permeabilization
No
Specification
Small intestine
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Fixative
Acetone
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/200
Secondary antibody
Dilution
1/300
Name
Non-Abcam antibody was used: Goat anti-Rabbit IgG (H+L) Cross-Adsorbed Secondar
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 546
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 546
Additional data
Additional Notes
Several dilutions have been tried: 1:50, 1:100, 1:200, 1:250, 1:500, 1:1000.
The result was the same: no staining found.
Abcam response
Thank you so much for testing this antibody in IHC-Fr. We appreciate very much your efforts, but are sorry to hear that the antibody did not work well in this application.
The customer received a discount on a future antibody purchase for testing this product. We welcome additional feedback from other customers as well.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 May 01 2018