Anti-ZO1 tight junction protein 抗体 (ab96587)
Key features and details
- Rabbit polyclonal to ZO1 tight junction protein
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-ZO1 tight junction protein antibody
ZO1 tight junction protein 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to ZO1 tight junction protein -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, WBmore details
適用なし: IHC-P -
種交差性
交差種: Mouse, Human -
免疫原
Recombinant protein fragment corresponding to a region within amino acids 1 - 266 of Human ZO1 tight junction protein.
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ポジティブ・コントロール
- WB: HEK-293T, HeLa, Neuro-2a, C8-D30, NIH/3T3 and C2C12 cell lysate, mouse testis tissue lysate. ICC/IF: NTERA-2 cl.D1 [NT2/D1] cells and H661 xenograft tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab96587の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
1/100 - 1/1000.
Fix cells in ice-cold MeOH |
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WB | (2) |
1/500 - 1/3000. Predicted molecular weight: 187 kDa.
5% gel. Blocking condition: 5% non-fat milk in TBST, RT, 60min. Primary antibody incubation: 4°C overnight.
Molecular weight shift may be caused by post-translation modification, there are multiple phosphorylation sites on ZO-1. It has been reported the detected molecular weight shifts to a higher size around 220-230 kDa (PMID: 26359454, PMID: 19117052). |
特記事項 |
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ICC/IF
1/100 - 1/1000. Fix cells in ice-cold MeOH |
WB
1/500 - 1/3000. Predicted molecular weight: 187 kDa. 5% gel. Blocking condition: 5% non-fat milk in TBST, RT, 60min. Primary antibody incubation: 4°C overnight.
Molecular weight shift may be caused by post-translation modification, there are multiple phosphorylation sites on ZO-1. It has been reported the detected molecular weight shifts to a higher size around 220-230 kDa (PMID: 26359454, PMID: 19117052). |
ターゲット情報
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機能
The N-terminal may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions. -
組織特異性
The alpha-containing isoform is found in most epithelial cell junctions. The short isoform is found both in endothelial cells and the highly specialized epithelial junctions of renal glomeruli and Sertoli cells of the seminiferous tubules. -
配列類似性
Belongs to the MAGUK family.
Contains 1 guanylate kinase-like domain.
Contains 3 PDZ (DHR) domains.
Contains 1 SH3 domain.
Contains 1 ZU5 domain. -
ドメイン
The second PDZ domain mediates interaction with GJA12. -
翻訳後修飾
Phosphorylated. Dephosphorylated by PTPRJ. -
細胞内局在
Cell membrane. Cell junction > tight junction. Movement of ZO-1 from the cytoplasm to membrane is an early event occurring concurrently with cell-cell contact. - Information by UniProt
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参照データベース
- Entrez Gene: 7082 Human
- Entrez Gene: 21872 Mouse
- Omim: 601009 Human
- SwissProt: Q07157 Human
- SwissProt: P39447 Mouse
- Unigene: 510833 Human
- Unigene: 4342 Mouse
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別名
- Tight junction protein 1 antibody
- Tight junction protein ZO-1 antibody
- Tight junction protein ZO1 antibody
see all
画像
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All lanes : Anti-ZO1 tight junction protein antibody (ab96587) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 187 kDa5% gel.
Running condition: 80V, 15min; 140V, 40min.
Transfer condition:Semi-dry, 18 V, 60min (Nitrocellulose membrane)
Blocking condition: 5% non-fat milk in TBST, RT, 60min.
Primary antibody incubation: 4°C overnight.
Secondary antibody incubation: Room temperature for 1 hour.
Washing condition: 5 ml TBST, 4 x 5min.
ECL detection.
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Immunofluorescence analysis of ice-cold methanol-fixed (5 min) NTERA-2 cl.D1 [NT2/D1] (human malignant pluripotent embryonic carcinoma cell line) cells labeling ZO1 tight junction protein at cell membrane (green) with ab96587 at 1/200 dilution. Blue: Hoechst 33342 staining.
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All lanes : Anti-ZO1 tight junction protein antibody (ab96587) at 1/500 dilution
Lane 1 : Neuro-2a (mouse neuroblastoma cell line) whole cell lysate
Lane 2 : C8-D30 (mouse astrocyte type III cerebellum cell line) whole cell lysate
Lane 3 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 187 kDa5% SDS-PAGE
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All lanes : Anti-ZO1 tight junction protein antibody (ab96587) at 1/1000 dilution
Lane 1 : Non-transfected HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
Lane 2 : ZO1 tight junction protein shRNA-transfected HeLa whole cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 187 kDa5% SDS-PAGE
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Anti-ZO1 tight junction protein antibody (ab96587) at 1/500 dilution + Mouse testis tissue lysate at 50 µg
Secondary
HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 187 kDa5% SDS-PAGE
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Anti-ZO1 tight junction protein antibody (ab96587) at 1/500 dilution + C2C12 (mouse myoblast cell line) whole cell lysate at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 187 kDa5% SDS-PAGE
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (152)
ab96587 は 152 報の論文で使用されています。
- Satitsri S et al. Piperine as potential therapy of post-weaning porcine diarrheas: an in vitro study using a porcine duodenal enteroid model. BMC Vet Res 19:4 (2023). PubMed: 36624444
- Nakamura A et al. The potential of soluble CD14 in discriminating nonalcoholic steatohepatitis from nonalcoholic fatty liver disease. Hepatol Res 52:508-521 (2022). PubMed: 35129841
- Nyandwi JB et al. Rosmarinic acid downregulates the oxLDL‑induced interaction between monocytes and endothelial cells, in addition to monocyte diapedesis, under high glucose conditions. Int J Mol Med 49:N/A (2022). PubMed: 35315501
- Xiong Y et al. Sodium Butyrate Attenuates Taurocholate-Induced Acute Pancreatitis by Maintaining Colonic Barrier and Regulating Gut Microorganisms in Mice. Front Physiol 13:813735 (2022). PubMed: 35370779
- Li DK et al. Exosomal HMGA2 protein from EBV-positive NPC cells destroys vascular endothelial barriers and induces endothelial-to-mesenchymal transition to promote metastasis. Cancer Gene Ther 29:1439-1451 (2022). PubMed: 35388172