Anti-ZNF148 抗体 (ab69933)
Key features and details
- Rabbit polyclonal to ZNF148
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-ZNF148 antibody
ZNF148 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to ZNF148 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human
交差が予測される動物種: Rat -
免疫原
Synthetic peptide (Human) from an internal region
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, 50% Glycerol (glycerin, glycerine), PBS
Without Mg2+ and Ca2+ -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab69933の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/1000. Detects a band of approximately 89 kDa (predicted molecular weight: 89 kDa).
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IHC-P | (1) |
1/50 - 1/100.
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特記事項 |
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WB
1/500 - 1/1000. Detects a band of approximately 89 kDa (predicted molecular weight: 89 kDa). |
IHC-P
1/50 - 1/100. |
ターゲット情報
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機能
Involved in transcriptional regulation. Represses the transcription of a number of genes including gastrin, stromelysin and enolase. Binds to the G-rich box in the enhancer region of these genes. -
配列類似性
Belongs to the krueppel C2H2-type zinc-finger protein family.
Contains 4 C2H2-type zinc fingers. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 7707 Human
- Entrez Gene: 58820 Rat
- Omim: 601897 Human
- SwissProt: Q9UQR1 Human
- SwissProt: Q62806 Rat
- Unigene: 592591 Human
- Unigene: 64671 Rat
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別名
- Transcription factor ZBP-89 antibody
- Transcription factor ZBP89 antibody
- ZBP89 antibody
see all
画像
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Immunohistochemical analysis of paraffin embedded human brain tissue using ab69933 at 1/50 dilution. Right panel was treated with immunising peptide.
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All lanes : Anti-ZNF148 antibody (ab69933) at 1/500 dilution
Lane 1 : HepG2 cell extract
Lane 2 : HepG2 cell extract with immunising peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 89 kDa
Observed band size: 89 kDa
Additional bands at: 95 kDa. We are unsure as to the identity of these extra bands.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (9)
ab69933 は 9 報の論文で使用されています。
- Qiu H et al. ZNF488 Promotes the Invasion and Migration of Pancreatic Carcinoma Cells through the Akt/mTOR Pathway. Comput Math Methods Med 2022:4622877 (2022). PubMed: 35111235
- Gao X et al. Upregulation of ZNF148 in SDHB-deficient gastrointestinal stromal tumor potentiates Forkhead box M1-mediated transcription and promotes tumor cell invasion. Cancer Sci 111:1266-1278 (2020). PubMed: 32060966
- Salmon M et al. ZFP148 (Zinc-Finger Protein 148) Binds Cooperatively With NF-1 (Neurofibromin 1) to Inhibit Smooth Muscle Marker Gene Expression During Abdominal Aortic Aneurysm Formation. Arterioscler Thromb Vasc Biol 39:73-88 (2019). PubMed: 30580567
- Salmon M et al. Klf4, Klf2, and Zfp148 activate autophagy-related genes in smooth muscle cells during aortic aneurysm formation. Physiol Rep 7:e14058 (2019). PubMed: 31025534
- Gao XH et al. ZNF148 modulates TOP2A expression and cell proliferation via ceRNA regulatory mechanism in colorectal cancer. Medicine (Baltimore) 96:e5845 (2017). PubMed: 28072746
- Zhang W et al. Upregulation of nemo-like kinase is an independent prognostic factor in colorectal cancer. World J Gastroenterol 21:8836-47 (2015). IHC-P ; Human . PubMed: 26269673
- Pourfarzad F et al. Locus-specific proteomics by TChP: targeted chromatin purification. Cell Rep 4:589-600 (2013). WB ; Human . PubMed: 23911284
- Gao XH et al. Expression of ZNF148 in different developing stages of colorectal cancer and its prognostic value: a large Chinese study based on tissue microarray. Cancer 119:2212-22 (2013). PubMed: 23576061
- Fanis P et al. Five friends of methylated chromatin target of protein-arginine-methyltransferase[prmt]-1 (chtop), a complex linking arginine methylation to desumoylation. Mol Cell Proteomics 11:1263-73 (2012). WB ; Mouse . PubMed: 22872859