Anti-ZCH11 抗体 (ab89165)
Key features and details
- Mouse polyclonal to ZCH11
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-ZCH11 antibody
ZCH11 一次抗体 製品一覧 -
製品の詳細
Mouse polyclonal to ZCH11 -
由来種
Mouse -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Recombinant full length protein within Human ZCH11. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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ポジティブ・コントロール
- Human spleen tissue lysate; ZCH11 transfected 293T cell lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab89165の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 185 kDa.
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特記事項 |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 185 kDa. |
ターゲット情報
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機能
Uridylyltransferase that acts as a suppressor of microRNA (miRNA) biogenesis by specifically mediating the terminal uridylation of some miRNAs. Catalyzes the 3' uridylation of precursor let-7 (pre-let-7), a miRNA precursor. Uridylated pre-let-7 miRNAs fail to be processed by Dicer and undergo degradation. Degradation of pre-let-7 contributes to the maintenance of embryonic stem (ES) cells and is required for ES cells to maintain pluripotency. Does not bind RNA by itself, recruited to pre-let-7 miRNAs via its interaction with LIN28A and LIN28B. Also catalyzes the 3' uridylation of miR-26A, a miRNA that represses IL6 transcript, leading to abrogate IL6 transcript repression and promote cytokine expression. May also suppress Toll-like receptor-induced NF-kappa-B activity via binding to T2BP. Does not play a role in replication-dependent histone mRNA degradation. -
配列類似性
Belongs to the DNA polymerase type-B-like family.
Contains 3 CCHC-type zinc fingers.
Contains 2 PAP-associated domains. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Nucleus. Cytoplasm. Translocates into the cytoplasm following treatment of the cell with LPS. - Information by UniProt
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参照データベース
- Entrez Gene: 23318 Human
- Omim: 613692 Human
- SwissProt: Q5TAX3 Human
- Unigene: 655407 Human
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別名
- DKFZp779C1943 antibody
- EC 2.7.7.52 antibody
- FLJ42878 antibody
see all
画像
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Anti-ZCH11 antibody (ab89165) at 1 µg/ml + Human spleen tissue lysate at 50 µg
Predicted band size: 185 kDa
Observed band size: 185 kDa -
All lanes : Anti-ZCH11 antibody (ab89165) at 1 µg/ml
Lane 1 : ZCH11 transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 185 kDa
データシートおよび資料
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Datasheet download
参考文献 (1)
ab89165 は 1 報の論文で使用されています。
- Plé H et al. The repertoire and features of human platelet microRNAs. PLoS One 7:e50746 (2012). WB ; Human . PubMed: 23226537