Anti-YAP1 (phospho Y357) 抗体 (ab62751)
Key features and details
- Rabbit polyclonal to YAP1 (phospho Y357)
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-YAP1 (phospho Y357) antibody
YAP1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to YAP1 (phospho Y357) -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Horse, Chicken, Cow, Dog, Xenopus laevis, Chimpanzee, Zebrafish, Opossum -
免疫原
Synthetic peptide corresponding to Human YAP1 aa 350-450 (C terminal) (phospho Y357) conjugated to keyhole limpet haemocyanin.
Database link: P46937 -
ポジティブ・コントロール
- HEK-293T cells co-transfected with human YAP1 and human c-Abl.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
バッファー
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab62751 was affinity-purified using the immunizing peptide immobilized on agarose. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab62751の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa).
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ICC/IF | (1) |
Use at an assay dependent concentration.
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特記事項 |
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WB
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 65 kDa (predicted molecular weight: 65 kDa). |
ICC/IF
Use at an assay dependent concentration. |
ターゲット情報
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機能
Transcriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Plays a key role to control cell proliferation in response to cell contact. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. The presence of TEAD transcription factors are required for it to stimulate gene expression, cell growth, anchorage-independent growth, and epithelial mesenchymal transition (EMT) induction. Isoform 2 and isoform 3 can activate the C-terminal fragment (CTF) of ERBB4 (isoform 3). -
組織特異性
Increased expression seen in some liver and prostate cancers. Isoforms lacking the transactivation domain found in striatal neurons of patients with Huntington disease (at protein level). -
配列類似性
Belongs to the YORKIE family.
Contains 2 WW domains. -
翻訳後修飾
Phosphorylated by LATS1 and LATS2; leading to cytoplasmic translocation and inactivation. Phosphorylated by ABL1; leading to YAP1 stabilization, enhanced interaction with TP73 and recruitment onto proapoptotic genes; in response to DNA damage. -
細胞内局在
Cytoplasm. Nucleus. Both phosphorylation and cell density can regulate its subcellular localization. Phosphorylation sequesters it in the cytoplasm by inhibiting its translocation into the nucleus. At low density, predominantly nuclear and is translocated to the cytoplasm at high density. - Information by UniProt
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参照データベース
- Entrez Gene: 396171 Chicken
- Entrez Gene: 479465 Dog
- Entrez Gene: 10413 Human
- Entrez Gene: 22601 Mouse
- Entrez Gene: 363014 Rat
- Entrez Gene: 561411 Zebrafish
- Omim: 606608 Human
- SwissProt: P46936 Chicken
see all -
別名
- 65 kDa Yes associated protein antibody
- 65 kDa Yes-associated protein antibody
- COB1 antibody
see all
画像
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All lanes : Anti-YAP1 (phospho Y357) antibody (ab62751) at 1/2000 dilution
Lane 1 : Whole cell lysates of HEK-293T cells co-transfected with human YAP1 and human c-Abl
Lane 2 : Whole cell lysates of untransfected HEK-293T cells
Lane 3 : Whole cell lysates of HEK-293T cells co-transfected with human YAP1 and human c-Abl with YAP1 peptide (human 351-362) at 20 µg/ml
Lane 4 : Whole cell lysates of HEK-293T cells co-transfected with human YAP1 and human c-Abl with phospho-YAP1 (human 351-362 [phospho Y357]) immunizing peptide at 20 µg/ml
Secondary
All lanes : Goat Anti-Rabbit IgG, Peroxidase conjugate
Predicted band size: 65 kDa
Observed band size: 65 kDa
Additional bands at: 100 kDa, 70 kDa. We are unsure as to the identity of these extra bands.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (47)
ab62751 は 47 報の論文で使用されています。
- Murphy R et al. Matrix Mechanotransduction via Yes-Associated Protein in Human Lamina Cribrosa Cells in Glaucoma. Invest Ophthalmol Vis Sci 63:16 (2022). PubMed: 35015027
- Guégan JP et al. Signaling by the tyrosine kinase Yes promotes liver cancer development. Sci Signal 15:eabj4743 (2022). PubMed: 35041461
- Chen T et al. α-Hederin Inhibits the Proliferation of Hepatocellular Carcinoma Cells via Hippo-Yes-Associated Protein Signaling Pathway. Front Oncol 12:839603 (2022). PubMed: 35311132
- Cheng N et al. Senescent hepatic stellate cells promote liver regeneration through IL-6 and ligands of CXCR2. JCI Insight 7:N/A (2022). PubMed: 35708907
- Sinnett-Smith J et al. Opposite Effects of Src Family Kinases on YAP and ERK Activation in Pancreatic Cancer Cells: Implications for Targeted Therapy. Mol Cancer Ther 21:1652-1662 (2022). PubMed: 35999654