Application
Western blot
Sample
Human Cell lysate - whole cell (293A)
Gel Running Conditions
Non-reduced Denaturing
Loading amount
10 µg
Specification
293A
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
1 hour(s) and 30 minute(s)
Dilution
1/8000
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-Rabbit HRP
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
5 second(s)
Bands
Specific: 75 kDa
Additional data
Additional Notes
Very excellent for WB.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Dec 21 2016