Anti-YAP1 (phospho S127) 抗体 [EP1675Y] - BSA and Azide free (ab172374)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1675Y] to YAP1 (phospho S127) - BSA and Azide free
- Suitable for: WB, IHC-P, Dot blot
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
-
製品名
Anti-YAP1 (phospho S127) antibody [EP1675Y] - BSA and Azide free
YAP1 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EP1675Y] to YAP1 (phospho S127) - BSA and Azide free -
由来種
Rabbit -
特異性
Stimulation may be required to allow detection of the phosphorylated protein. Please see images below for recommended treatment conditions and positive controls.
-
アプリケーション
適用あり: WB, IHC-P, Dot blotmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
-
特記事項
ab172374 is the carrier-free version of ab76252.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading...
-
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP1675Y -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
- Alexa Fluor® 488 Anti-YAP1 (phospho S127) antibody [EP1675Y] (ab310956)
- Alexa Fluor® 647 Anti-YAP1 (phospho S127) antibody [EP1675Y] (ab311073)
- Alexa Fluor® 568 Anti-YAP1 (phospho S127) antibody [EP1675Y] (ab312934)
- Alexa Fluor® 555 Anti-YAP1 (phospho S127) antibody [EP1675Y] (ab313146)
- Anti-YAP1 (phospho S127) antibody [EP1675Y] (ab76252)
-
Conjugation kits
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab172374の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
Use at an assay dependent concentration. Detects a band of approximately 65 kDa (predicted molecular weight: 65-75 kDa).
|
|
IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
|
|
Dot blot |
Use at an assay dependent concentration.
|
特記事項 |
---|
WB
Use at an assay dependent concentration. Detects a band of approximately 65 kDa (predicted molecular weight: 65-75 kDa). |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Dot blot
Use at an assay dependent concentration. |
ターゲット情報
-
機能
Transcriptional regulator which can act both as a coactivator and a corepressor and is the critical downstream regulatory target in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Plays a key role to control cell proliferation in response to cell contact. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. The presence of TEAD transcription factors are required for it to stimulate gene expression, cell growth, anchorage-independent growth, and epithelial mesenchymal transition (EMT) induction. Isoform 2 and isoform 3 can activate the C-terminal fragment (CTF) of ERBB4 (isoform 3). -
組織特異性
Increased expression seen in some liver and prostate cancers. Isoforms lacking the transactivation domain found in striatal neurons of patients with Huntington disease (at protein level). -
配列類似性
Belongs to the YORKIE family.
Contains 2 WW domains. -
翻訳後修飾
Phosphorylated by LATS1 and LATS2; leading to cytoplasmic translocation and inactivation. Phosphorylated by ABL1; leading to YAP1 stabilization, enhanced interaction with TP73 and recruitment onto proapoptotic genes; in response to DNA damage. -
細胞内局在
Cytoplasm. Nucleus. Both phosphorylation and cell density can regulate its subcellular localization. Phosphorylation sequesters it in the cytoplasm by inhibiting its translocation into the nucleus. At low density, predominantly nuclear and is translocated to the cytoplasm at high density. - Information by UniProt
-
参照データベース
- Entrez Gene: 10413 Human
- Entrez Gene: 22601 Mouse
- Entrez Gene: 363014 Rat
- Omim: 606608 Human
- SwissProt: P46937 Human
- SwissProt: P46938 Mouse
- SwissProt: Q2EJA0 Rat
- Unigene: 503692 Human
see all -
別名
- 65 kDa Yes associated protein antibody
- 65 kDa Yes-associated protein antibody
- COB1 antibody
see all
画像
-
Dot blot analysis of YAP1 (pS127) peptide (Lane 1) and YAP1 non-phospho peptide (Lane 2) labelling YAP1 (pS127) with ab76252 at a dilution of 1/1000. ab97051 (Peroxidase conjugated goat anti-rabbit IgG (H+L)) was used as the secondary antibody at a dilution of 1/100000.
Blocking and dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76252).
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat stomach tissue labelling YAP1 (phospho S127) with purified ab76252 at 1/250. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76252).
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue labelling YAP1 (phospho S127) with purified ab76252 at 1/250. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76252).
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human adenocarcinoma of endometrium tissue labelling YAP1 (phospho S127) with purified ab76252 at 1/250. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab76252).
プロトコール
データシートおよび資料
-
Datasheet download
参考文献 (3)
ab172374 は 3 報の論文で使用されています。
- Zhao L et al. lncRNA MALAT1 modulates cancer stem cell properties of liver cancer cells by regulating YAP1 expression via miR-375 sponging. Mol Med Rep 22:1449-1457 (2020). PubMed: 32626943
- Zhang C et al. AMOT130 linking F-actin to YAP is involved in intervertebral disc degeneration. Cell Prolif N/A:e12492 (2018). PubMed: 30039887
- Zhang C et al. Dysregulation of YAP by the Hippo pathway is involved in intervertebral disc degeneration, cell contact inhibition, and cell senescence. Oncotarget 9:2175-2192 (2018). PubMed: 29416763