Anti-XRN2 抗体 (ab72284)
Key features and details
- Rabbit polyclonal to XRN2
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
-
製品名
Anti-XRN2 antibody
XRN2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to XRN2 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan, Elephant -
免疫原
Synthetic peptide corresponding to a region between residues 25 and 75 of human XRN2 (NP_036387.2).
-
ポジティブ・コントロール
- Whole cell lysates from HeLa, 293T and NIH3T3 cells.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
-
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab72284の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
1/2000 - 1/10000. Detects a band of approximately 109, 115 kDa (predicted molecular weight: 109 kDa).
|
特記事項 |
---|
WB
1/2000 - 1/10000. Detects a band of approximately 109, 115 kDa (predicted molecular weight: 109 kDa). |
ターゲット情報
-
関連性
XRN2 possesses 5'->3' exoribonuclease activity. XRN2 shares similarity with the mouse Dhm1 and the yeast dhp1 gene. The function of the human gene has not yet been determined, however, it may promote the termination of transcription by RNA polymerase II. During transcription termination, cleavage at the polyadenylation site liberates a 5' fragment which is subsequently processed to form the mature mRNA and a 3' fragment which remains attached to the elongating polymerase. The processive degradation of this 3' fragment by this protein may promote termination of transcription. -
細胞内局在
Nucleus, nucleolus. -
参照データベース
- Entrez Gene: 22803 Human
- Entrez Gene: 24128 Mouse
- Entrez Gene: 362229 Rat
- Omim: 608851 Human
- SwissProt: Q9H0D6 Human
- SwissProt: Q9DBR1 Mouse
-
別名
- 5' 3' exoribonuclease 2 antibody
- DHM1 like protein antibody
- DHP protein antibody
- XRN 2 antibody
画像
-
All lanes : Anti-XRN2 antibody (ab72284) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Predicted band size: 109 kDa
Observed band size: 109,115 kDa why is the actual band size different from the predicted?
Additional bands at: 55 kDa. We are unsure as to the identity of these extra bands.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (2)
ab72284 は 2 報の論文で使用されています。
- Rother S et al. NF-?B-repressing factor phosphorylation regulates transcription elongation via its interactions with 5'?3' exoribonuclease 2 and negative elongation factor. FASEB J 30:174-85 (2016). PubMed: 26340924
- Ternette N et al. Label-free quantitative proteomics reveals regulation of interferon-induced protein with tetratricopeptide repeats 3 (IFIT3) and 5'-3'-exoribonuclease 2 (XRN2) during respiratory syncytial virus infection. Virol J 8:442 (2011). WB ; Human . PubMed: 21933386