Application
ChIP
Sample
Mouse Cell lysate - whole cell (G1E)
Negative control
ChIP XRN2 on ALAS2 TSS and ChiP IgG
Specification
G1E
Detection step
Real-time PCR
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 32 minute(s) and 0 second(s)
Specification of the cross-linking agent: EGS then Formaldehyde
Duration of cross-linking step: 32 minute(s) and 0 second(s)
Specification of the cross-linking agent: EGS then Formaldehyde
Positive control
ChIP XRN2 from another vendor on TAL1 TTS
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Dilution buffer
Concentration
0.2 µg/µg chromatin
Additional data
Abcam response
Thank you for your valuable feedback. We have not previously tested this antibody in ChIP and therefore it may not be suitable for use in this application. Further optimization may improve results.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Dec 22 2016