Anti-XRCC4 抗体 (ab97351)
Key features and details
- Rabbit polyclonal to XRCC4
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-XRCC4 antibody
XRCC4 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to XRCC4 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WB, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide, corresponding to a region within amino acids 266-279 of Human XRCC4 (NP_071801)
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ポジティブ・コントロール
- WB: HeLa cell nuclear extract. IHC-P: HBL435 xenograft tissue. IF: HeLa cell.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab97351の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
1/100 - 1/1000.
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WB | (1) |
1/500 - 1/3000. Detects a band of approximately 55 kDa (predicted molecular weight: 38 kDa).
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ICC/IF | (2) |
1/100 - 1/1000.
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特記事項 |
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IHC-P
1/100 - 1/1000. |
WB
1/500 - 1/3000. Detects a band of approximately 55 kDa (predicted molecular weight: 38 kDa). |
ICC/IF
1/100 - 1/1000. |
ターゲット情報
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機能
Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. Binds to DNA and to DNA ligase IV (LIG4). The LIG4-XRCC4 complex is responsible for the NHEJ ligation step, and XRCC4 enhances the joining activity of LIG4. Binding of the LIG4-XRCC4 complex to DNA ends is dependent on the assembly of the DNA-dependent protein kinase complex DNA-PK to these DNA ends. -
組織特異性
Widely expressed. -
配列類似性
Belongs to the XRCC4 family. -
翻訳後修飾
Phosphorylated by PRKDC. The phosphorylation seems not to be necessary for binding to DNA. Phosphorylation by CK2 promotes interaction with APTX.
Monoubiquitinated.
Sumoylation at Lys-210 is required for nuclear localization and recombination efficiency. Has no effect on ubiquitination. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 7518 Human
- Omim: 194363 Human
- SwissProt: Q13426 Human
- Unigene: 567359 Human
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別名
- DNA double strand break repair and V(D)J recombination protein XRCC4 antibody
- DNA repair protein XRCC4 antibody
- SSMED antibody
see all
画像
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All lanes : Anti-XRCC4 antibody (ab97351) at 1/500 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HeLa cell nuclear extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 38 kDa10% SDS-PAGE
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Immunohistochemical analysis of paraffin-embedded HBL435 xenograft using ab97351 at 1/500 dilution.
Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
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Immunofluorescence analysis of paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labelling XRCC4 using ab97351 at 1/200 dilution. Lower image was also labelled with DNA probe.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab97351 は 5 報の論文で使用されています。
- Francica P et al. Functional Radiogenetic Profiling Implicates ERCC6L2 in Non-homologous End Joining. Cell Rep 32:108068 (2020). PubMed: 32846126
- Deraska PV et al. NF-?B inhibition by dimethylaminoparthenolide radiosensitizes non-small-cell lung carcinoma by blocking DNA double-strand break repair. Cell Death Discov 4:10 (2018). PubMed: 29531807
- Willis NA et al. Rad51 recruitment and exclusion of non-homologous end joining during homologous recombination at a Tus/Ter mammalian replication fork barrier. PLoS Genet 14:e1007486 (2018). PubMed: 30024881
- Stelcer E et al. Chondrocytes differentiated from human induced pluripotent stem cells: Response to ionizing radiation. PLoS One 13:e0205691 (2018). PubMed: 30352062
- Willis NA et al. Mechanism of tandem duplication formation in BRCA1-mutant cells. Nature 551:590-595 (2017). PubMed: 29168504