Anti-XPD 抗体 (ab111596)
Key features and details
- Rabbit polyclonal to XPD
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-XPD antibody
XPD 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to XPD -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Cow -
免疫原
Recombinant fragment, corresponding to a region within amino acids 34-381 of Human XPD (NP_000391).
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ポジティブ・コントロール
- NT2D1, IMR32, U-87 MG and MCF7 cells and whole cell lysates; Human Breast carcinoma tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab111596 is purified by antigen affinity chromatography. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab111596の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 87 kDa.
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IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternative antigen retrieval method: Tris-EDTA buffer pH 8.0
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ICC/IF |
1/100 - 1/500.
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特記事項 |
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WB
1/500 - 1/3000. Predicted molecular weight: 87 kDa. |
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternative antigen retrieval method: Tris-EDTA buffer pH 8.0 |
ICC/IF
1/100 - 1/500. |
ターゲット情報
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機能
ATP-dependent 5'-3' DNA helicase, component of the core-TFIIH basal transcription factor. Involved in nucleotide excision repair (NER) of DNA by opening DNA around the damage, and in RNA transcription by RNA polymerase II by anchoring the CDK-activating kinase (CAK) complex, composed of CDK7, cyclin H and MAT1, to the core-TFIIH complex. Involved in the regulation of vitamin-D receptor activity. As part of the mitotic spindle-associated MMXD complex it plays a role in chromosome segregation. Might have a role in aging process and could play a causative role in the generation of skin cancers. -
関連疾患
Defects in ERCC2 are the cause of xeroderma pigmentosum complementation group D (XP-D) [MIM:278730]; also known as XP group D (XPD). Xeroderma pigmentosum is an autosomal recessive pigmentary skin disorder characterized by solar hypersensitivity of the skin, high predisposition for developing cancers on areas exposed to sunlight and, in some cases, neurological abnormalities. Some XP-D patients present features of Cockayne syndrome, including dwarfism, sensorineural deafness, microcephaly, mental retardation, pigmentary retinopathy, ataxia, decreased nerve conduction velocities.
Defects in ERCC2 are a cause of trichothiodystrophy photosensitive (TTDP) [MIM:601675]. TTDP is an autosomal recessive disease characterized by sulfur-deficient brittle hair and nails, ichthyosis, mental retardation, impaired sexual development, abnormal facies and cutaneous photosensitivity correlated with a nucleotide excision repair (NER) defect. Neonates with trichothiodystrophy and ichthyosis are usually born with a collodion membrane. The severity of the ichthyosis after the membrane is shed is variable, ranging from a mild to severe lamellar ichthyotic phenotype. There are no reports of skin cancer associated with TTDP.
Defects in ERCC2 are the cause of cerebro-oculo-facio-skeletal syndrome type 2 (COFS2) [MIM:610756]. COFS is a degenerative autosomal recessive disorder of prenatal onset affecting the brain, eye and spinal cord. After birth, it leads to brain atrophy, hypoplasia of the corpus callosum, hypotonia, cataracts, microcornea, optic atrophy, progressive joint contractures and growth failure. Facial dysmorphism is a constant feature. Abnormalities of the skull, eyes, limbs, heart and kidney also occur. -
配列類似性
Belongs to the helicase family. RAD3/XPD subfamily.
Contains 1 helicase ATP-binding domain. -
翻訳後修飾
ISGylated. -
細胞内局在
Nucleus. Cytoplasm > cytoskeleton > spindle. - Information by UniProt
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参照データベース
- Entrez Gene: 100125238 Cow
- Entrez Gene: 2068 Human
- Entrez Gene: 13871 Mouse
- Entrez Gene: 308415 Rat
- Omim: 278730 Human
- SwissProt: A6QLJ0 Cow
- SwissProt: P18074 Human
- SwissProt: O08811 Mouse
see all -
別名
- TFIIH 80 kDa subunit antibody
- Basic transcription factor 2 80 kDa subunit antibody
- BTF2 p80 antibody
see all
画像
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Anti-XPD antibody (ab111596) at 1/1000 dilution + MCF7 whole cell lysate at 30 µg
Predicted band size: 87 kDa
7.5% SDS-PAGE. -
ab111596 at 1/500 dilution staining XPD in Paraformaldehyde-fixed MCF7 cells by Immunofluorescence. Lower image shows cells co-stained with Hoechst 33342.
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ab111596 at range of 1/100- 1/1000 dilution staining XPD in paraffin-embedded Human Breast carcinoma tissue by Immunohistochemistry.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab111596 は 1 報の論文で使用されています。
- Huang MY et al. ERCC overexpression associated with a poor response of cT4b colorectal cancer with FOLFOX-based neoadjuvant concurrent chemoradiation. Oncol Lett 20:212 (2020). PubMed: 32963618