Anti-WDR48/UAF1 抗体 (ab122473)
Key features and details
- Rabbit polyclonal to WDR48/UAF1
- Suitable for: IHC-P, ICC
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-WDR48/UAF1 antibody
WDR48/UAF1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to WDR48/UAF1 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, ICCmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment corresponding to Human WDR48/UAF1 aa 350-450 (internal sequence).
Database link: Q8TAF3 -
ポジティブ・コントロール
- IHC-P: Human testis, endometrium, cerebral cortex, and cervix tissues; ICC: A431 cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab122473の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC |
Use a concentration of 0.25 - 2 µg/ml.
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特記事項 |
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IHC-P
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC
Use a concentration of 0.25 - 2 µg/ml. |
ターゲット情報
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機能
Regulator of deubiquitinating complexes. Acts as a strong activator of USP1 by enhancing the USP1-mediated deubiquitination of FANCD2; USP1 being almost inactive by itself. Also activates deubiquitinating activity of complexes containing USP12 and USP46, respectively. Activates deubiquitination by increasing the catalytic turnover without increasing the affinity of deubiquitinating enzymes for the substrate. In case of infection by Herpesvirus saimiri, may play a role in vesicular transport or membrane fusion events necessary for transport to lysosomes. Induces lysosomal vesicle formation via interaction with Herpesvirus saimiri tyrosine kinase-interacting protein (TIP). Subsequently, TIP recruits tyrosine-protein kinase LCK, resulting in down-regulation of T-cell antigen receptor TCR. May play a role in generation of enlarged endosomal vesicles via interaction with TIP. In case of infection by papillomavirus HPV11, promotes the maintenance of the viral genome via its interaction with HPV11 helicase E1. -
組織特異性
Ubiquitous. -
配列類似性
Belongs to the WD repeat WDR48 family.
Contains 8 WD repeats. -
ドメイン
N-terminal WD region interacts with TIP and C-terminal region mediates lysosomal localization. The WD repeats are required for the interaction with deubiquitinating enzymes USP1, USP12 and USP46. -
細胞内局在
Nucleus. Cytoplasm. Lysosome. Mainly cytoplasmic. In case of infection by papillomavirus HPV11, translocates to the nucleus via its interaction with papillomavirus HPV11. - Information by UniProt
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参照データベース
- Entrez Gene: 57599 Human
- Omim: 612167 Human
- SwissProt: Q8TAF3 Human
- Unigene: 109778 Human
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別名
- p80 antibody
- UAF 1 antibody
- UAF1 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testis tissue labelling WDR48/UAF1 with ab122473 at 1/50 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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ab122473, at 4 µg/ml, staining WDR48/UAF1 in PFA/Triton X-100 fixed/permeabilized A431 cells by Immunofluorescence (green).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human endometrium tissue labelling WDR48/UAF1 with ab122473 at 1/50 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebral cortex tissue labelling WDR48/UAF1 with ab122473 at 1/50 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cervix tissue labelling WDR48/UAF1 with ab122473 at 1/50 dilution. Heat mediated antigen retrieval performed with citrate buffer pH 6 before commencing with IHC staining protocol.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab122473 は 4 報の論文で使用されています。
- Jang SW & Kim JM Mutation of aspartic acid 199 in USP1 disrupts its deubiquitinating activity and impairs DNA repair. FEBS Lett 595:1997-2006 (2021). PubMed: 34128540
- Song H et al. UAF1 deubiquitinase complexes facilitate NLRP3 inflammasome activation by promoting NLRP3 expression. Nat Commun 11:6042 (2020). PubMed: 33247121
- Yu Z et al. USP1-UAF1 deubiquitinase complex stabilizes TBK1 and enhances antiviral responses. J Exp Med 214:3553-3563 (2017). PubMed: 29138248
- Gabriel CH et al. Identification of Novel Nuclear Factor of Activated T Cell (NFAT)-associated Proteins in T Cells. J Biol Chem 291:24172-24187 (2016). PubMed: 27637333