製品の概要
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製品名Anti-WAPL antibody
WAPL 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to WAPL
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由来種Rabbit
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アプリケーション適用あり: WBmore details
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種交差性交差種: Human
交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Rhesus monkey, Gorilla, Platypus
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免疫原
Synthetic peptide corresponding to a region between residues 1125 and 1175 of human WAPL (SwissProt entry Q7Z5K2)
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ポジティブ･コントロール
- HeLa nuclear extract.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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バッファーPreservative: 0.09% Sodium Azide
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8 -
Concentration information loading... -
精製度Immunogen affinity purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab70741 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | 1/2500 - 1/10000. Detects a band of approximately 175 kDa (predicted molecular weight: 133 kDa). |
ターゲット情報
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機能Regulator of sister chromatid cohesion in mitosis which negatively regulates cohesin association with chromatin. Involved in both sister chromatid cohesion during interphase and sister-chromatid resolution during early stages of mitosis. Couples DNA replication to sister chromatid cohesion. Cohesion ensures that chromosome partitioning is accurate in both meiotic and mitotic cells and plays an important role in DNA repair.
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組織特異性Isoform 1 is highly expressed in uterine cervix tumor. Isoform 2 is widely expressed with a high level in skeletal muscle and heart.
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配列類似性Contains 1 WAPL domain.
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細胞内局在Nucleus and Nucleus. Chromosome. Cytoplasm. Associates with chromatin through the cohesin complex during interphase. Released in the cytoplasm from nuclear envelope breakdown until anaphase, it reaccumulates in nucleus at telophase.
- Information by UniProt
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参照データベース
- Entrez Gene: 23063 Human
- Entrez Gene: 218914 Mouse
- Entrez Gene: 290577 Rat
- Omim: 610754 Human
- SwissProt: Q7Z5K2 Human
- SwissProt: Q65Z40 Mouse
- Unigene: 203099 Human
- Unigene: 714876 Human
see all -
別名
- FOE antibody
- Friend of EBNA2 (Epstein Barr virus nuclear protein 2) antibody
- Friend of EBNA2 protein antibody
see all
画像
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Western blot - WAPL antibody (ab70741)All lanes : Anti-WAPL antibody (ab70741) at 0.2 µg/ml
Lane 1 : HeLa nuclear extract at 5 µg
Lane 2 : HeLa nuclear extract at 20 µg
Predicted band size: 133 kDa
Observed band size: 175 kDa (why is the actual band size different from the predicted?)
Exposure time: 20 minutes -
Western blot - Anti-WAPL antibody (ab70741)All lanes : Anti-WAPL antibody (ab70741) at 1/2000 dilution
Lane 1 : Human U2OS cells
Lane 2 : Human U2OS cells treated with siRNA #1 against WAPL.
Lane 3 : Human U2OS cells treated with siRNA #2 against WAPL.
Lysates/proteins at 90 µg per lane.
Secondary
All lanes : Immunopure Pierce anti-rabbit HRP conjugated at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 133 kDa
Observed band size: 150 kDa (why is the actual band size different from the predicted?)
プロトコール
参考文献
This product has been referenced in:
- Stelloh C et al. The cohesin-associated protein Wapal is required for proper Polycomb-mediated gene silencing. Epigenetics Chromatin 9:14 (2016). Read more (PubMed: 27087855) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"