Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Rat Tissue sections (Spinal cord)
Antigen retrieval step
None
Permeabilization
Yes - Triton-X100
Specification
Spinal cord
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 26°C
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS
Dilution
1/200
Secondary antibody
Dilution
1/500
Name
Non-Abcam antibody was used: Alexa Fluor 488-conjugated goat anti-rabbit antibo
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. K. A.
Verified customer
投稿 Sep 30 2016