Anti-Viral Protein U 抗体 (ab81532)
Key features and details
- Rabbit polyclonal to Viral Protein U
- Suitable for: ELISA, WB, ICC/IF, IP
- Reacts with: Human immunodeficiency virus
- Isotype: IgG
製品の概要
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製品名
Anti-Viral Protein U antibody -
製品の詳細
Rabbit polyclonal to Viral Protein U -
由来種
Rabbit -
アプリケーション
適用あり: ELISA, WB, ICC/IF, IPmore details -
種交差性
交差種: Human immunodeficiency virus -
免疫原
Synthetic peptide corresponding to Human immunodeficiency virus Viral Protein U aa 50-150 conjugated to keyhole limpet haemocyanin.
Database link: Q6L8A2 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
Preservative: 0.02% Sodium azide
Proprietary antibody stabilization buffer. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab81532の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ELISA |
1/50000.
|
|
WB |
1/1000. Positive for Cells transfected with Vpu
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|
ICC/IF |
1/250.
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|
IP |
1/250.
|
特記事項 |
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ELISA
1/50000. |
WB
1/1000. Positive for Cells transfected with Vpu |
ICC/IF
1/250. |
IP
1/250. |
ターゲット情報
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関連性
Retroviruses have several characteristic structural and catalytic proteins. One such auxiliary protein is a viral protein U (Vpu) which enhances virion release from human cells and is also involved in the degradation of CD4, the cellular surface receptor of HIV-1. The Vpu has no homolog in less pathogenic HIV-2 virus. Vpu is an 81 amino acid class I membrane integral protein that is unique to human and simian immunodeficiency virus isolated from Chimpanzee and a few other monkey species. The 16kDa protein Vpu protein consists of an N-terminal hydrophobic membrane anchor of 27 amino acids and a charged C-terminal hydrophilic domain of 54 amino acids that extents to the cytoplasm. The cytoplasmic domain has a conserved dual serine phosphorylation site (S52 GXX and S56 motif) that is phosphorylated by casein kinase II. Vpu is involved in viral replication and degradation of its cellular receptor CD4 and enhancement of viral particle release from macrophages and primary lymphocytes. -
細胞内局在
Membrane; Single-pass type I membrane protein. -
別名
- U ORF protein antibody
- Vpu antibody
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (2)
ab81532 は 2 報の論文で使用されています。
- Triantafilou K et al. Differential recognition of HIV-stimulated IL-1ß and IL-18 secretion through NLR and NAIP signalling in monocyte-derived macrophages. PLoS Pathog 17:e1009417 (2021). PubMed: 33861800
- Swann J et al. Cytosolic sulfotransferase 1A1 regulates HIV-1 minus-strand DNA elongation in primary human monocyte-derived macrophages. Virol J 13:30 (2016). PubMed: 26906565