Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Cornea epithelial Cells)
Specification
Cornea epithelial Cells
Fixative
Methanol
Permeabilization
Yes - 0.2% Triton-X 15mins
Blocking step
BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 28°C
Other product details
Dilution
1/100
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Secondary antibody
Name
Non-Abcam antibody was used: Alexa Fluro 633 anti-CK polyclonal conjugate
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 647
Additional data
Additional Notes
Samples was prepared through cytospin section from the same donor. Positive control vimentin(ab16700)were used. Secondary antibody is used for the negative control. The ab39376 antibody gives out lots of background compare with the positive control
Abcam response
Thank you for your honest feedback regarding your experience using this antibody. It is possible that further dilution of the primary antibody would reduce the background you see.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MISS. Ashley Quintana
Verified customer
投稿 Aug 17 2010