Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Non-reduced Non-Denaturing (Native) (12,5)
Sample
Human Tissue lysate - whole (human Urether)
Specification
human Urether
Blocking step
Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBS + Tween
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: anti Mouse, Sigma A2554
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/50000
Detection
Detection method
ECL
Bands
Specific: 54 kDa
Exposure
3 minute(s) and 0 second(s)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Nov 11 2013