Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Human Limbal Stromal cells)
Specification
Human Limbal Stromal cells
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 2%
Other product details
Dilution
1/200
Incubation time
1 hour(s) and 0 minute(s)
Secondary antibody
Name
Non-Abcam antibody was used: Sigma Anti-Rabbit
Host species: Goat
Clonality: Monoclonal
Conjugation: Fluorescein
Host species: Goat
Clonality: Monoclonal
Conjugation: Fluorescein
Additional data
Additional Notes
Green = Vimentin Positive (FITC)
Blue = Hoechst Nuclear Staining
The upper cells (Vimentin Negative) are epithelium cells
Vimentin Positive cells are the stroma cells
Blue = Hoechst Nuclear Staining
The upper cells (Vimentin Negative) are epithelium cells
Vimentin Positive cells are the stroma cells
Abcam response
ICC/IF was entered for "application" but based on the information provided in this Abreview, I believe this should be marked as IHC.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MISS. Ashley Quintana
Verified customer
投稿 Nov 20 2007