Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Testis, adult)
Permeabilization
Yes - 0.1% Triton X-100 in PBS
Specification
Testis, adult
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Paraformaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 20°C
Dilution
1/10
Secondary antibody
Dilution
1/500
Name
Abcam antibody:Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 555) preadsorbed
Additional data
Additional Notes
We have tried the antibody at dilutions at 1:10, 1:50 1:100, 1:250, 1:500, and 1:1000, but were unable to detect a clear signal above backround level. DAPI (blue) was used as a counterstain.
Abcam response
Thank you for sharing this data with us. ab16700 has previously been shown to work in IHC-Fr on human tissues (please see the IHC-Fr image and references on our datasheet). However, based on the data shown here, it is clear that ab16700 is not suitable for IHC-Fr on mouse tissues. We would welcome feedback from other researchers currently using this antibody.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Bryan Niedenberger
Verified customer
投稿 Jul 12 2016