Application
Western blot
Sample
Human Tissue lysate - other (Aorta)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
5 µg
Treatment
PNGaseF
Specification
Aorta
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA
Dilution
1/2000
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: Jackson ImmunoResearch
Host species: Mouse
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Mouse
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
30 second(s)
Bands
Specific: 65, 55 kDa
Additional data
Additional Notes
Right sample was treated with PNGaseF.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Javier Barallobre-Barreiro
Verified customer
投稿 Jun 24 2015