Application
Western blot
Sample
Pig Tissue lysate - whole (brain (hypothalamus))
Gel Running Conditions
Reduced Denaturing (12)
Loading amount
60 µg
Specification
brain (hypothalamus)
Blocking step
BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
Other product details
Incubation time
36 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST
Dilution
1/200
Secondary antibody
Dilution
1/12000
Name
Non-Abcam antibody was used: Goat anti-rabbit IgG h+l - Bethyl
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
SuperSignal West Dura kit
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 17 kDa Non-specific: 25, 28, 35 kDa
Positive control
Hypothalamus - Preoptic area, ventral hypothalamus, dorsal hypothalamus
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投稿 Jul 04 2017