Anti-VSV-G tag antibody (ab1874)

  Immunohistochemistry (Frozen sections) - VSV-G tag antibody (ab1874)

ab1874 detecting bound HS scFv antibody in rat lung tissue by Immunohistochemistry (Frozen sections).

Lungs from Sprague-Dawley rats were harvested at embryonic days 17.5 of gestation and fixed with 4% (w/v) paraformaldehyde in phosphate-buffered saline (PBS), pH 7.4. The lungs were washed in PBS, cryoprotected with 20% (w/v) sucrose in PBS, and gelatin-embedded (7.5% (w/v) gelatin, 15% (w/v) sucrose in PBS) before being covered in Cryo-M-Bed and snap frozen at -40 °C. The lung sections were cut at 8 µm and mounted onto chrome alum gel slides for storage at -40 °C. Tissue sections were blocked with 10% (v/v) goat serum in PBS and incubated overnight at 4 °C with HS scFv antibody, diluted 1/5 in 1% (v/v) goat serum in PBS. Bound antibody was detected with ab1874 diluted 1/200 in 1% (v/v) goat serum in PBS, followed by fluorescein isothiocyanate-conjugated goat anti-rabbit IgG.

aw, airway; me, mesenchyme; bm, basement membrane.

Image from Thompson SM et al, J Biol Chem. 2009 Dec 18;284(51):35621-31, Fig 1. DOI 10.1074/jbc.M109.009712