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Anti-VR1 antibody (ab63083)

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Overview

Product name

Anti-VR1 antibody
VR1 抗体 (24件) 一覧

Description

Rabbit polyclonal to VR1

Tested applications

ICC/IF, WB, IHC-P, IHC-Frmore details

Cross reactivity

Reacts with

Rat, Human

Immunogen

Synthetic peptide as a part of human VR1 conjugated to an immunogenic carrier protein.

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C (add glycerol to a final volume of 50% for extra stability). Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: Whole serum

Purity

Whole antiserum

Clonality

Polyclonal

Isotype

IgG

Research areas

Neuroscience >> Sensory System >> Somatosensory system >> Nociception
Neuroscience >> Neurotransmission >> Receptors / Channels >> More Ion Channels

  • Immunocytochemistry/ Immunofluorescence - VR1 antibody (ab63083)Immunocytochemistry/ Immunofluorescence - VR1 antibody (ab63083) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab63083 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • 1 Image

    ICC/IF

     ICC/IF: 1/1000.

    ICC/IF: 1/1000.

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    WB

     WB: 1/300 - 1/3000. Pred...Read more →

    WB: 1/300 - 1/3000. Predicted molecular weight: 95 kDa.This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.

  • IHC-P

     IHC-P: 1/300 - 1/3000.

    IHC-P: 1/300 - 1/3000.

  • IHC-Fr

     IHC-Fr: 1/300 - 1/3000.

    IHC-Fr: 1/300 - 1/3000.

Target

Function

Receptor-activated non-selective calcium permeant cation channel involved in detection of noxious chemical and thermal stimuli. Seems to mediate proton influx and may be involved in intracellular acidosis in nociceptive neurons. May be involved in mediation of inflammatory pain and hyperalgesia. Sensitized by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases, which involves PKC isozymes and PCL. Acts as ionotropic endocannabinoid receptor with central neuromodulatory effects. Triggers a form of long-term depression (TRPV1-LTD) mediated by the endocannabinoid anandamine in the hippocampus and nucleus accubens by affecting AMPA receptors endocytosis.

Tissue specificity

Widely expressed at low levels. Expression is elevated in dorsal root ganglia. In skin, expressed in cutaneous sensory nerve fibers, mast cells, epidermal keratinocytes, dermal blood vessels, the inner root sheet and the infundibulum of hair follicles, differentiated sebocytes, sweat gland ducts, and the secretory portion of eccrine sweat glands (at protein level).

Sequence similarities

Belongs to the transient receptor (TC 1.A.4) family. TrpV subfamily. TRPV1 sub-subfamily.
Contains 6 ANK repeats.

Domain

The association domain (AD) is necessary for self-association.

Post-translational
modifications

Phosphorylation by PKA reverses capsaicin-induced dephosphorylation at multiple sites, probably including Ser-117 as a major phosphorylation site. Phoshphorylation by CAMKII seems to regulate binding to vanilloids. Phosphorylated and modulated by PKCM and probably PKCZ. Dephosphorylation by calcineurin seems to lead to receptor desensitization and phosphorylation by CAMKII recovers activity.

Cellular localization

Cell junction > synapse > postsynaptic cell membrane. Cell projection > dendritic spine membrane.

Target information above from: UniProt accessionQ8NER1 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • capsaicin receptor antibody
  • DKFZp434K0220 antibody
  • osm 9 like TRP channel 1 antibody
  • Osm-9-like TRP channel 1 antibody
  • OTRPC1 antibody
  • Transient receptor potential cation channel subfamily V member 1 antibody
  • TRPV 1 antibody
  • TRPV1 antibody
  • TRPV1_HUMAN antibody
  • Vanilloid receptor 1 antibody
  • Vanilloid receptor subtype 1 antibody
  • VR 1 antibody
  • VR1 antibody
see all

Database links

Anti-VR1 antibody images:

  Immunocytochemistry/ Immunofluorescence - VR1 antibody (ab63083)

Immunocytochemistry/ Immunofluorescence - VR1 antibody (ab63083)

ICC/IF image of ab63083 stained SHSY5Y cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab63083, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References for Anti-VR1 antibody (ab63083)

This product has been referenced in:

  • Lu Y  et al. Trichostatin A, a histone deacetylase inhibitor, reduces lesion growth and hyperalgesia in experimentally induced endometriosis in mice. Hum Reprod 25:1014-25 (2010). IHC-P.Read more (PubMed: 20118114) »

See 1 publication for this product

Publishing research using ab63083? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"