Application
Western blot
Sample
Pig Tissue lysate - whole (Pig heart (left ventricle))
Loading amount
50 µg
Specification
Pig heart (left ventricle)
Gel Running Conditions
Reduced Denaturing (3-8% Novex Tris-Acetate)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Dilution
1/300
Incubation time
10 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 3% BSA in TBST (0.05% Tween 20)
Secondary antibody
Name
Non-Abcam antibody was used: 711-035-152 (Jackson ImmunoResearch)
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/8000
Detection
Detection method
ECL
Exposure
3 minute(s) and 0 second(s)
Bands
Specific: 140, 180 kDa Non-specific: >250 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Apr 09 2012