Anti-VEGF Receptor 2 抗体 [mV1001.3m-h] (ab42228)
Key features and details
- Mouse monoclonal [mV1001.3m-h] to VEGF Receptor 2
- Suitable for: Sandwich ELISA, WB
- Reacts with: Human, Recombinant fragment
- Isotype: IgG1
製品の概要
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製品名
Anti-VEGF Receptor 2 antibody [mV1001.3m-h]
VEGF Receptor 2 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [mV1001.3m-h] to VEGF Receptor 2 -
由来種
Mouse -
アプリケーション
適用あり: Sandwich ELISA, WBmore details -
種交差性
交差種: Human, Recombinant fragment -
免疫原
Purified fragment, corresponding to N terminal amino acids 30-200 of Human VEGF Receptor 2
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ポジティブ・コントロール
- Human Umbilical Vein Endothelial Cells (HUVECs).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Avoid freeze / thaw cycle. -
バッファー
Constituent: PBS -
Concentration information loading...
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精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
mV1001.3m-h -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
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sELISA pair antibody
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab42228の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Sandwich ELISA |
Use a concentration of 5 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to VEGF Receptor 2 (ab11939).
For sandwich ELISA, use this antibody as Capture at 5µg/ml with ab11939 as Detection. |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 151 kDa.
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特記事項 |
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Sandwich ELISA
Use a concentration of 5 µg/ml. Can be paired for Sandwich ELISA with Rabbit polyclonal to VEGF Receptor 2 (ab11939). For sandwich ELISA, use this antibody as Capture at 5µg/ml with ab11939 as Detection. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 151 kDa. |
ターゲット情報
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機能
Receptor for VEGF or VEGFC. Has a tyrosine-protein kinase activity. The VEGF-kinase ligand/receptor signaling system plays a key role in vascular development and regulation of vascular permeability. In case of HIV-1 infection, the interaction with extracellular viral Tat protein seems to enhance angiogenesis in Kaposi's sarcoma lesions. -
関連疾患
Defects in KDR are associated with susceptibility to hemangioma capillary infantile (HCI) [MIM:602089]. HCI are benign, highly proliferative lesions involving aberrant localized growth of capillary endothelium. They are the most common tumor of infancy, occurring in up to 10% of all births. Hemangiomas tend to appear shortly after birth and show rapid neonatal growth for up to 12 months characterized by endothelial hypercellularity and increased numbers of mast cells. This phase is followed by slow involution at a rate of about 10% per year and replacement by fibrofatty stroma. -
配列類似性
Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
Contains 7 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 protein kinase domain. -
翻訳後修飾
Phosphorylated. Dephosphorylated by PTPRB. Dephosphorylated by PTPRJ at Tyr-951, Tyr-996, Tyr-1054, Tyr-1059, Tyr-1175 and Tyr-1214. -
細胞内局在
Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 3791 Human
- Omim: 191306 Human
- SwissProt: P35968 Human
- Unigene: 479756 Human
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別名
- CD309 antibody
- CD309 antigen antibody
- EC 2.7.10.1 antibody
see all
画像
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Lane 1 : unstimulated cells
Lane 2 : hVEGF at 20 ng/ml
Lane 3 : Anti-VEGF Receptor 2 antibody [mV1001.3m-h] (ab42228) at 5 µg/ml
Lane 4 : Anti-VEGF Receptor 2 antibody [mV1001.3m-h] (ab42228) at 25 µg/ml
All lanes : HUVECs
Predicted band size: 151 kDa
Observed band size: 220 kDa why is the actual band size different from the predicted?
HUVECs (human umbilical vein endothelial cells) were stimulated with hVEGF or ab42228 for 30min. Phospho-VEGF Receptor 2 was detected with IP-Western for Phosphotyrosine.
データシートおよび資料
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Datasheet download
参考文献 (4)
ab42228 は 4 報の論文で使用されています。
- Silva JAF et al. Spatial and temporal VEGF receptor intracellular trafficking in microvascular and macrovascular endothelial cells. Sci Rep 11:17400 (2021). PubMed: 34462507
- Peng F et al. PFKFB3 is involved in breast cancer proliferation, migration, invasion and angiogenesis. Int J Oncol 52:945-954 (2018). PubMed: 29393396
- Liu H et al. Human umbilical vein endothelial cell vaccine suppresses the angiogenesis of esophageal squamous cell carcinoma in a humanized mouse model. Oncol Rep 40:3006-3014 (2018). PubMed: 30132560
- Ptaszynska MM et al. Positive Feedback between Vascular Endothelial Growth Factor-A and Autotaxin in Ovarian Cancer Cells. Mol Cancer Res 6:352-63 (2008). PubMed: 18337445