Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer, pH 6
Sample
Mouse Tissue sections (15 days old WT embryonic brain, 16 micron)
Specification
15 days old WT embryonic brain, 16 micron
Permeabilization
Yes - Triton X100
Fixative
Formaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: PBS with 0.1% Triton X100, 1% serum
Dilution
1/300
Secondary antibody
Name
Non-Abcam antibody was used: Donkey anti-Rabbit IgG (H+L) Secondary Antibody, A
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/500
Additional data
Additional Notes
Antigen-retrieval was carried out in 10mM citrate buffer, pH 6, at 97 degree for 20 min prior to staining. Signal was largely derived from choroid plexus as shown in the images. (Higher magnification was shown in the lower row.)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Mar 06 2015