Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (primary rat asqtrocytes)
Permeabilization
No
Specification
primary rat asqtrocytes
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 23°C
Fixative
Methanol
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 23°C · Diluent: 1/100
Secondary antibody
Name
Non-Abcam antibody was used: Jackson Immuno Research
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
I tested this ab in both primary rat astrocytes and eahy926 (ibrid cell line HUVEC and Human lung carcinoma).
In both cell line it did not work, both with pfa and metoh fixation.
Abcam response
The end user has tested this antibody under Abtrial offer with new untested Rat species It seems like the antibody hasn't work as predicted. Customer has been offered troubleshooting and they have Abtrial code which they can use to get next antibody free of charge.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Lavinia Capuana
Verified customer
投稿 Sep 23 2016