Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Human Brain Microvascular Endothelial Cells)
Permeabilization
Yes - 0.1% Triton in PBS
Specification
Human Brain Microvascular Endothelial Cells
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative
Paraformaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: Blocking agent
Dilution
1/200
Secondary antibody
Dilution
1/500
Name
Non-Abcam antibody was used: Anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jan 27 2016