Application
Western blot
Sample
Pig Tissue lysate - other (protein lysate from lymph node)
Loading amount
30 µg
Specification
protein lysate from lymph node
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
Other product details
Dilution
1/250
Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS-T
Secondary antibody
Name
Non-Abcam antibody was used: A0545 Sigma, anti-rabbit IgG
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL+
Exposure
3 minute(s) and 0 second(s)
Positive control
Protein lysate fron human PBL
Additional data
Additional Notes
1. What did you mean by Immobilon?
- Immobilon (PVDF)
2. Was the image completely blank? If not could you please upload it?
- The antibody has been tested in a separate blot
- Immobilon (PVDF)
2. Was the image completely blank? If not could you please upload it?
- The antibody has been tested in a separate blot
Abcam response
We are thankful for customer's feedback and collaboration on testing the antibody in pig samples.
This is untested species but looking at Human result, the antibody is working.
Adding blocking agents to the antibody dilution buffers and using more secondary should improve the results.
This is untested species but looking at Human result, the antibody is working.
Adding blocking agents to the antibody dilution buffers and using more secondary should improve the results.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MS. Angela Moreno
Verified customer
投稿 Mar 04 2011