Application
Western blot
Sample
Spermophilus tridecemlineatus Tissue lysate - other (Liver tissue fractions)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
20 µg
Specification
Liver tissue fractions
Blocking step
BSA as blocking agent for 8 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
Other product details
Incubation time
15 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST with 0.3% BSA
Dilution
1/4000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: Pierce (Thermo) #31460, Goat anti-rabbit, IgG, HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL+
Negative control
Liver cytosolic fraction
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 30.7 kDa Non-specific: many kDa
Positive control
Liver whole tissue and mitochondrial fraction
Additional data
Additional Notes
Lane1=liver whole tissue homogenate
Lane2=liver cytosolic fraction
Lane3=liver mitochondrial fraction
Abcam response
Thank you very much for your valuable feedback. We are pleased to see, that this antibody is working well in Spermophilus tridecemlineatus, a species that has not been tested before.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. James Bjork
Verified customer
投稿 Nov 03 2015