Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% TritonX100 in PBS
Other product details
Dilution
1/200
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: PBS
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-Rabbit Alexafluor488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/200
Additional data
Additional Notes
Asynchronous HeLa were paraformaldehyde-fixed, permeablized, immunofluorescently labeled and counterstained with DAPI. This antibody produces a focal staining pattern that is enriched within interphase nuclei and therefore does not appear to produce the expected staining pattern. Methanol fixed samples were also evaluated and failed to produce the expected staining pattern.
Abcam response
Thank you for your valuable feedback. ICC/IF was not a tested application for ab36047 and it seems that this antibody does not recognize VCP under these experimental conditions. Thank you for your time - we hope that this information will be helpful for future customers.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Kirk Mcmanus
Verified customer
投稿 Aug 03 2011