Application
Western blot
Loading amount
15 µg
Gel Running Conditions
Reduced Denaturing (gel 10 %)
Sample
Plants Cell lysate - whole cell (tobacco cells in suspension)
Specification
tobacco cells in suspension
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 20°C
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: TBS Tween 0.1 %, BSA 2 %
Dilution
1/1000
Secondary antibody
Name
Non-Abcam antibody was used: anti-Rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/20000
Detection
Detection method
ECL
Bands
Specific: 90 kDa Non-specific: 35 (weak) kDa
Exposure
5 minute(s) and 0 second(s)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MS. Claire Rosnoblet
Verified customer
投稿 Oct 15 2013