Application
Western blot
Sample
Human Cell lysate - whole cell (HT29 cells)
Loading amount
20 µg
Specification
HT29 cells
Treatment
EPA for 24 hrs
Gel Running Conditions
Reduced Denaturing (10% bis tris gel)
Blocking step
Thermo scientific superblock buffer in TBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 24°C
Other product details
Dilution
1/20000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: superblock blocking buffer in TBS
Secondary antibody
Name
Non-Abcam antibody was used: anti-rabbit IgG
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/100000
Detection
Detection method
ecl+
Exposure
4 minute(s) and 0 second(s)
Bands
Specific: 100 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Joanne Doleman
Verified customer
投稿 Oct 22 2008