Anti-VAV1 抗体 (ab97574)
Key features and details
- Rabbit polyclonal to VAV1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-VAV1 antibody
VAV1 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to VAV1 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Mouse, Human -
免疫原
Recombinant protein fragment containing a sequence corresponding to a region within amino acids 83 and 538 of Human VAV1 (NP_005419)
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ポジティブ・コントロール
- WB: Jurkat, NCI-H929 and BCL-1 whole cell lysate. IHC-P: Human serous ovarian cancer.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab97574の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 98 kDa.
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IHC-P |
1/100 - 1/1000.
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特記事項 |
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WB
1/500 - 1/3000. Predicted molecular weight: 98 kDa. |
IHC-P
1/100 - 1/1000. |
ターゲット情報
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機能
Couples tyrosine kinase signals with the activation of the Rho/Rac GTPases, thus leading to cell differentiation and/or proliferation. -
組織特異性
Widely expressed in hematopoietic cells but not in other cell types. -
配列類似性
Contains 1 CH (calponin-homology) domain.
Contains 1 DH (DBL-homology) domain.
Contains 1 PH domain.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 SH2 domain.
Contains 2 SH3 domains. -
ドメイン
The DH domain is involved in interaction with CCPG1. -
翻訳後修飾
Phosphorylated on tyrosine residues. - Information by UniProt
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参照データベース
- Entrez Gene: 7409 Human
- Entrez Gene: 22324 Mouse
- Omim: 164875 Human
- SwissProt: P15498 Human
- SwissProt: P27870 Mouse
- Unigene: 116237 Human
- Unigene: 248172 Mouse
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別名
- Oncogene vav antibody
- p95Vav antibody
- Proto-oncogene vav antibody
see all
画像
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All lanes : Anti-VAV1 antibody (ab97574) at 1/500 dilution
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2 : Raji (human Burkitt's lymphoma cell line) whole cell lysate
Lane 3 : NCI-H929 (human bone marrow lymphoblast cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 98 kDa7.5% SDS-PAGE
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Paraffin-embedded human serous ovarian cancer tissue stained VAV1 using ab97574 at 1/100 dilution in immunohistochemical analysis.
Antigen Retrieval: EDTA based buffer, pH 8.0, 15min
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Anti-VAV1 antibody (ab97574) at 1/1000 dilution + BCL-1 (mouse B lymphocyte lymphoma cell line) whole cell lysate at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 98 kDa7.5% SDS-PAGE
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All lanes : Anti-VAV1 antibody (ab97574) at 1/500 dilution
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 2 : NCI-H1299 (human lung carcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 98 kDa7.5% SDS-PAGE
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (7)
ab97574 は 7 報の論文で使用されています。
- Zhu X et al. TMT-Based Quantitative Proteomic Analysis Reveals Downregulation of ITGAL and Syk by the Effects of Cycloastragenol in OVA-Induced Asthmatic Mice. Oxid Med Cell Longev 2022:6842530 (2022). PubMed: 36329800
- He B et al. MicroRNA-326 decreases tau phosphorylation and neuron apoptosis through inhibition of the JNK signaling pathway by targeting VAV1 in Alzheimer's disease. J Cell Physiol 235:480-493 (2020). PubMed: 31385301
- Liu Y et al. Stearic acid methyl ester ?promotes migration of mesenchymal stem cells and accelerates cartilage defect repair. J Orthop Translat 22:81-91 (2020). PubMed: 32440503
- Li Y et al. Human iPSC-Derived Natural Killer Cells Engineered with Chimeric Antigen Receptors Enhance Anti-tumor Activity. Cell Stem Cell 23:181-192.e5 (2018). PubMed: 30082067
- Zhou Y et al. Protective effects of microRNA-330 on amyloid β-protein production, oxidative stress, and mitochondrial dysfunction in Alzheimer's disease by targeting VAV1 via the MAPK signaling pathway. J Cell Biochem 119:5437-5448 (2018). PubMed: 29369410
- Kang L et al. Elevated level of Vav1 was correlated with advanced biological behavior and poor prognosis in patients with gastric cancer. Int J Clin Exp Pathol 11:391-398 (2018). PubMed: 31938123
- Boddicker RL et al. Integrated mate-pair and RNA sequencing identifies novel, targetable gene fusions in peripheral T-cell lymphoma. Blood 128:1234-45 (2016). PubMed: 27297792