V5 tag peptide (ab15829)
Key features and details
- Purity: > 90% HPLC
- Suitable for: Blocking
製品の詳細
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製品名
V5 tag peptide -
精製度
> 90 % HPLC. -
アクセッション番号
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Animal free
No -
由来
Synthetic -
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生物種
Parainfluenza virus 5
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関連製品
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Corresponding Antibody
特性
Our Abpromise guarantee covers the use of ab15829 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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アプリケーション
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製品の状態
Lyophilized -
備考
- First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
- If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
- Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
- Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
- Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use. -
Concentration information loading...
前処理および保存
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保存方法および安定性
Shipped at 4°C. Store at -20°C.
Information available upon request.
関連情報
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別名
- GKPIPNPLLGLDST epitope tag
- GKPIPNPLLGLDST tag
- Protein Rev
see all -
関連性
The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).
画像
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Lane 1: Primary: ab15828, 0.5 ug/ml
Sample: Recombinant V5 tagged Groucho homolog, 20 ug
Secondary: Goat anti-Rabbit IgG, 1/10000 dilutionLane 2: Primary: ab15828, 0.5 ug/ml
Blocking peptide: ab15829, 1 ug/ml
Sample: Recombinant V5 tagged Groucho , 20 ug
Secondary: Goat anti-Rabbit IgG, 1/10000 dilution
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
参考文献 (2)
ab15829 は 2 報の論文で使用されています。
- Emery A et al. Target identification for small-molecule discovery in the FOXO3a tumor-suppressor pathway using a biodiverse peptide library. Cell Chem Biol 28:1602-1615.e9 (2021). PubMed: 34111400
- Dou K et al. Drosophila P75 safeguards oogenesis by preventing H3K9me2 spreading. J Genet Genomics 47:187-199 (2020). PubMed: 32499180