V5 tag antibody (ab9137)

Product Name 

V5 tag antibody

Product type 

Primary antibodies

Description 

Goat polyclonal to V5 tag

Immunogen 

Synthetic peptide: GKPIPNPLLGLDST (V5) conjugated to KLH.
BLAST 'GKPIPNPLLGLDST' with or

Specificity 

Antibody was verified by ELISA against peptide conjugated to BSA(GKPIPNPLLGLDST/BSA).

Epitope 

GKPIPNPLLGLDST

Tested applications 
(see key)

ELISA, ICC, WB

Abreviews 

Customer reviews (see key)

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Application notes 
(see key)

Recommended dilutions
ELISA: Use at a dilution of: Coating: 1:100 - 1:500
Primary: 1:1000 - 1:30,000.
ICC: 1/200 - 1/2000.
WB: 1/1000 - 1/30000.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Nuclear

Research areas 

Tags & Cell Markers >> Epitope Tags >> V5 Tag

Relevance 

The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).

Raised in 

Goat

Clonality 

Polyclonal

Purity 

Immunogen affinity purified

Storage buffer 

PBS, pH 7.2, sodium azide, 0.1%

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

This antibody was affinity purified using the peptide immobilized on solid support.
Antibody concentration was determined by extinction coefficient: absorbance at280 nm of 1.4 equals 1.0 mg of IgG.

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C.

References for V5 tag antibody (ab9137)

  This product has been used in: (two most recent references)

Wong CC et al. High-efficiency stem cell fusion-mediated assay reveals Sall4 as an enhancer of reprogramming. PLoS ONE 3:e1955 (2008). WB. PubMed: 18414659

Odegrip R et al. CIS display: In vitro selection of peptides from libraries of protein-DNA complexes. Proc Natl Acad Sci U S A 101:2806-10 (2004). PubMed: 14981246
If you publish research using ab9137 please let us know so that we can cite the reference on this datasheet.

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