Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Tags & Cell Markers >> Epitope Tags >> V5 Tag
Overview
Product name
Anti-V5 tag antibody
V5 tag 抗体 (24件) 一覧
Description
Goat polyclonal to V5 tag
Specificity
Antibody was verified by ELISA against peptide conjugated to BSA(GKPIPNPLLGLDST/BSA).
Tested applications
WB, ELISA, ICCmore details
Immunogen
Synthetic peptide: GKPIPNPLLGLDST (V5) conjugated to KLH.
GKPIPNPLLG LDST
Epitope
GKPIPNPLLGLDST
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
PBS, pH 7.2, sodium azide, 0.1%
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
This antibody was affinity purified using the peptide immobilized on solid support. Antibody concentration was determined by extinction coefficient: absorbance at280 nm of 1.4 equals 1.0 mg of IgG.
Clonality
Polyclonal
Isotype
unknown
Light chain type
unknown
Research areas
Applications
Show applications key
Our Abpromise guarantee covers the use of ab9137 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
ELISA
ICC
Application notes
ELISA: Use at a dilution of: Coating: 1:100 - 1:500
Primary: 1:1000 - 1:30,000.
ICC: 1/200 - 1/2000.
WB: 1/1000 - 1/30000.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).
Cellular localization
Not applicable
Alternative names
- GKPIPNPLLGLDST epitope tag antibody
- GKPIPNPLLGLDST tag antibody
- Protein Rev antibody
see all
References for Anti-V5 tag antibody (ab9137)
This product has been referenced in:
- Wong CCet al. High-efficiency stem cell fusion-mediated assay reveals Sall4 as an enhancer of reprogramming. PLoS ONE 3:e1955 (2008). WB.Read more (PubMed: 18414659) »
- Odegrip Ret al. CIS display: In vitro selection of peptides from libraries of protein-DNA complexes. Proc Natl Acad Sci U S A 101:2806-10 (2004).Read more (PubMed: 14981246) »
See all 2 publications for this product
Publishing research using ab9137? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"