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Products:Tags & Cell Markers >> Epitope Tags >> V5 Tag
Overview
Product name
Anti-V5 tag antibody
V5 tag 抗体 (24件) 一覧
Description
Chicken polyclonal to V5 tag
Specificity
Antibody specificity was verified by ELISA against the peptide conjugated to BSA (GKPIPNPLLGLDST-BSA). A 1:100,000 dilution of the antibody gave an O.D.=1.0 in a 15 minute reaction using HRP conjugated goat anti chicken IgY (IgG) and TMB as the substrate. Appropriate specificity controls were run.
Tested applications
ELISA, WB, ICC/IF, ICCmore details
Immunogen
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
PBS, pH 7.2, sodium azide, 0.1%
Concentration
Concentration information loading...
Purity
IgY fraction
Purification notes
Antibodies were immunoaffinity purified using the peptide immobilized on a solid support. Antibody concentration was determined by extinction coefficient : O.D. 1.32 at 280nM equals 1.0 mg of IgY (IgG).
Clonality
Polyclonal
Isotype
unknown
Light chain type
unknown
Research areas
-
Immunocytochemistry/ Immunofluorescence - V5 tag antibody (ab9113)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab9113 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: Use at an assay dependent dilution.
WB
WB: 1/5000WB: 1/5000
ICC/IF: Use at an assay dependent dilution.
ICC: Use at an assay dependent dilution.
Target
Relevance
The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).
Cellular localization
Not applicable
Alternative names
- GKPIPNPLLGLDST epitope tag antibody
- GKPIPNPLLGLDST tag antibody
- Protein Rev antibody
see all
Anti-V5 tag antibody images:
Immunocytochemistry/ Immunofluorescence - V5 tag antibody (ab9113)
ab9113 staining human fibrosarcoma cells (HT1080) by ICC/IF. Cells were transfected with V5- tagged transmembrane protein and PFA fixed without undergoing permeabilization and blocking steps. The primary antibody was used at 10µg/ml and incubated with the sample for 1 hour at 20°C. An Alexa Fluor® 546 conjugated donkey polyclonal anti-chicken (H+L) antibody, diluted 1/500, was used as the secondary.
This image is courtesy of an anonymous Abreview
References for Anti-V5 tag antibody (ab9113)
This product has been referenced in:
- Stanton RJet al. Reconstruction of the complete human cytomegalovirus genome in a BAC reveals RL13 to be a potent inhibitor of replication. J Clin Invest 120:3191-208 (2010). WB.Read more (PubMed: 20679731) »
See 1 publication for this product
Publishing research using ab9113? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"