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Immunocytochemistry/ Immunofluorescence - V5 tag antibody (ab9113)
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Product Name
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V5 tag antibody
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V5 tag 抗体 (22件) 一覧
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Product type
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Primary antibodies
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Description
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Chicken polyclonal to V5 tag
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Immunogen
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GKPIPNPLLGLDST (V5 epitope) conjugated with KLH.
BLAST 'GKPIPNPLLGLDST' with  or 
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Specificity
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Antibody specificity was verified by ELISA against the peptide conjugated to BSA (GKPIPNPLLGLDST-BSA).
A 1:100,000 dilution of the antibody gave an O.D.=1.0 in a 15 minute reaction using HRP conjugated goat anti chicken IgY (IgG) and TMB as the substrate.
Appropriate specificity controls were run.
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Tested applications
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ELISA, ICC/IF, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: Use at an assay dependent dilution.
ICC: Use at an assay dependent dilution.
ICC/IF: Use at an assay dependent dilution.
WB: 1/5000.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Nuclear
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Research areas
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Tags & Cell Markers >> Epitope Tags >> V5 Tag
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Relevance
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The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).
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V5 tag antibody (ab9113)の別名 |
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Raised in
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Chicken
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Clonality
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Polyclonal
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Purity
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IgY fraction
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Storage buffer
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PBS, pH 7.2, sodium azide, 0.1% Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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Antibodies were immunoaffinity purified using the peptide immobilized on a solid support. Antibody concentration was determined by extinction coefficient : O.D. 1.32 at 280nM equals 1.0 mg of IgY (IgG).
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C.
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Abcam では全ての製品情報を一元管理しており、 V5 tag antibody について私たちが知りうる全ての情報がこのデータシートでご覧いただけます。ご不明な点がありましたら、 お問い合わせください。 |
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See below for V5 tag antibody images, references, products related to ab9113 and other tools.
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V5 tag antibody images:
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 Immunocytochemistry/ Immunofluorescence - V5 tag antibody (ab9113)
ab9113 staining human fibrosarcoma cells (HT1080) by ICC/IF. Cells were transfected with V5- tagged transmembrane protein and PFA fixed without undergoing permeabilization and blocking steps. The primary antibody was used at 10µg/ml and incubated with the sample for 1 hour at 20°C. An Alexa Fluor® 546 conjugated donkey polyclonal anti-chicken (H+L) antibody, diluted 1/500, was used as the secondary. This image is courtesy of an anonymous Abreview
See Abreview
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Search PubMed (MEDLINE) for references to V5 tag
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V5 tag antibody - more information
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Customer reviews (feedback) regarding V5 tag antibody |
Customer FAQs regarding V5 tag antibody |
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Price and availability of products related to V5 tag antibody |
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V5 tag antibody - related products:
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V5 tag antibody - other tools:
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Contact Abcam with a Technical Enquiry about V5 tag antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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