V5 tag antibody (ab84164)

Product Name 

V5 tag antibody

Product type 

Primary antibodies

Description 

Sheep polyclonal to V5 tag

Immunogen 

Synthetic peptide: GKPIPNPLLGLDST, corresponding to amino acids 95-108 of V5 tag (Simian virus 5, strain W3) conjugated to an immunogenic carrier protein.
BLAST 'GKPIPNPLLGLDST' with or

Specificity 

Reacts with V5 (GKPIPNPLLGLDST) expressed in any organism.

Tested applications 
(see key)

IHC-Fr, IHC-P, WB

Abreviews 

Customer reviews (see key)

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Application notes 
(see key)

Recommended dilutions
IHC-P: 1/300 - 1/2000. Antigen retrieval is recommended.
IHC-Fr: 1/300 - 1/2000.
WB: 1/300 - 1/2000.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Nuclear

Research areas 

Tags & Cell Markers >> Epitope Tags >> V5 Tag

Relevance 

The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus 5 (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).

Raised in 

Sheep

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Whole antiserum

Storage buffer 

Preservative: None
Constituents: PBS, Whole serum

Form 

Liquid

Concentration 

Concentration not determined - その理由

Storage instructions 

Store at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50%, aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

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