Anti-V5 tag antibody (ab84164)

I appreciate a lot your informative and helpful reply. The customer decided to proceed with the abreview promotion of ab9116 as you mentioned for IHC-Fr (following our correspondence below). Please issue the discount code ASAP, because the customer would like to add this antibody to our order that will be shipped tomorrow (this Friday). Thanks in advance for your reply. Happy Holidays,

ANSWER:

DISCOUNT CODE: XXXXXX Expiration date: 20-Apr-2012

I am very pleased to hear you would like to accept our offer and test ab9116 in IHC-FR (4% PFA fixed Drosophila sections). This code will give you 1 free [PRIMARY ANTIBODY] before the expiration date.

To redeem this offer, please submit an Abreview for IHC-FR and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative.

If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Thank you for the mail. As I mentioned in my previous mail the customer works with Drosophila BRAIN (fresh brain) that is fixed with 4% paraformaldehyde for 10 min. The customer does not perform any antigen retrieval. As this customer explained me this type of the staining that he performs is very similar to the staining of the cells.   Please advise if the following antibodies will be covered by Abcam's abpromise or you advise different choice. If these are not covered by Abpromise will it be possible to proceed with the Abreview promotion (the most urgent is ab98795). http://www.abcam.com/V5-tag-antibody-ChIP-Grade-ab9116.html http://www.abcam.com/V5-tag-antibody-FITC-ab1274.html http://www.abcam.com/V5-tag-antibody-ab84164.html http://www.abcam.com/HA-tag-antibody-ab9134.html http://www.abcam.com/Donkey-polyclonal-Secondary-Antibody-to-Mouse-IgG-H-L-DyLight-550-pre-adsorbed-ab98795.html Thank you an advance.   Kind Regards,  

ANSWER:

Thank you for contacting us. ab1274, ab9116 and ab98795 are not covered under Abpromise guarantee as per customer request. I am including other product under IHC-Fr application with PFA as fixative. Ab98795 is a secondary antibody; Abreview discount code is not valid for this product. However if customer provide us Abreview we will assign the Abpoints by manually publishing the Abreview. You can forward the Abreview to me. The Abreview discount code for ab1274 and ab9116 can be provided however customer needs to explain how they have expressed tag conjugated protein in drosophila; please also forward the end user details. Please be also advised that ab1274, ab9116, ab9134, ab84164 are the antibodies against tags. These tags are not naturally present in any cells. These products can only be used in detecting the tag conjugated recombinant proteins that are expressed in tissues or cells. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Dear Tech Support Team, Kindly advise if the following antibodies   http://www.abcam.com/V5-tag-antibody-ChIP-Grade-ab9116.html   http://www.abcam.com/V5-tag-antibody-FITC-ab1274.html   http://www.abcam.com/V5-tag-antibody-ab84164.html   http://www.abcam.com/HA-tag-antibody-ab9134.html   http://www.abcam.com/Donkey-polyclonal-Secondary-Antibody-to-Mouse-IgG-H-L-DyLight-550-pre-adsorbed-ab98795.html   are covered by Abpromise for the immunohistostaining of Drosophila brain if the customer treats the brain in the following way:   10 min in 4% paraformaldehyde and proceeds with the staining.   What is the abbreviation that fits this type of the treatment?   Thanks in advance for your assistance and reply.   Have a nice week,

ANSWER:

Thank you for contacting us.

These antibodies are against tags so species is not applicable for these products.

"10 min in 4% paraformaldehyde and proceeds with the staining" is not enough information that we need for any recommendations. Please ask the customer to provide following info

- Are they planning to use tissue sections or cells? What type of tissue section or cells they will be using e.g. Eye, Brain etc. - Are they planning Immunohistochemistry on frozen sections or paraformaldehyde sections thereby incubating sections with acetone or ethanol or treating sections with antigen retrieval method? - Now these antibodies are against tags so you may ask how they are expressing the tag in cells or tissues.

Please provide the above information then only we can provide further confirmation.