Application
Western blot
Sample
Mouse Cell lysate - whole cell (NIH3T3 cells)
Gel Running Conditions
Reduced Denaturing (4%-12%)
Loading amount
20 µg
Treatment
Transfected with or without V5-tagged plasmids
Specification
NIH3T3 cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST with 2.5%BSA
Dilution
1/1000
Secondary antibody
Dilution
1/3000
Name
Non-Abcam antibody was used: Polyclonal Rabbit Anti Mouse Immunoglobulins/HRP
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Negative control
Cells were not transfected with V5-tagged plasmid.
Exposure
1 second(s)
Bands
Specific: 28 kDa
Positive control
Cells were transfected a V5-tagged HSPB8 (MW:~25kD)plasmid, which expressed a V5-tagged protein with molecular weight of approximately 28kD.
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投稿 Jul 25 2016